FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727556
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25210.14592869927226673No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC24360.14100845356098443No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC20530.11883840523838302No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC19890.11513374964400576Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA19800.11461278245104645Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC19750.11432335623273572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT43450.028.1925567
GATCTTT49700.026.6205336
TCTTTTT45600.026.3260368
CTTTTTT46500.025.7412349
GTGTGTA15900.021.79374970
CGATCTT109200.019.8735525
GTGTACA2002.4272595E-819.261741
TTTCGGA4650.018.828681
ATCTGGG18500.018.7292187
CAGGGGT6800.018.5315884
GATCTGG47500.018.4216166
AGTGTGT19650.018.16902269
TACACAG4100.017.9284155
TAGGGGT6300.017.7798654
TAGATAC3800.017.501555
GATATGC78750.017.4232670
GGCGTTA5050.017.32776564
CGTTAAC4650.017.31285966
GATTAGG1854.7250414E-617.037411
ACTAGGT1452.4369489E-416.8995153
Produced by FastQC (version 0.11.5)