FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397796
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG17680.12648483755855647No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17050.12197774210256718No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT16480.11789989383286259No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA15130.10824183214145698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGC2650.030.3763038
CTAGGCC1401.2732926E-1129.9989599
TAGGCCC1056.7656583E-726.672424
TCTAGGG3950.024.8208082
ATCTGGG13500.024.1111357
CGATCTG89500.024.0546075
GATCTGG31150.023.820256
CTAGGCG1052.0415846E-523.3325239
ATCTGAG7400.023.17577
TAGGGGT5900.022.5472374
CCGATCT221800.022.5228924
CTATACT2350.022.3452434
TTAGGCC1102.9248287E-522.27195210
TTCCGAT224350.022.2560672
TCCGATC227050.022.0183093
CTTCCGA232650.021.7685261
GGCCGGG5000.021.6984712
CTAGTCT850.003786433320.5926764
CGATCTT37100.020.5697385
TCTGACC3250.020.4608278
Produced by FastQC (version 0.11.5)