FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTGAAGCGTGCTTCCCGCGTAGATGCTTTCAGCGGTTATCACAGCCC10.6993006993006993No Hit
TCTCCCTGCAAAGATCGGAAGAGCACACGTCTCCCTGCAAAGATCGGAAG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTAGTGCGCATGGCTAGCGCGACTGGTTCCTCTGTCACCTTGGTTACTCT10.6993006993006993No Hit
GACGAATGTAGCTCATTCGCGACTGATAAACCCTCTAGACATCCACCGAC10.6993006993006993No Hit
GAGTTGGGTGGGGCGGGGGGGTGTGCGGGTGACTCGCGGGCCCGCCCGTC10.6993006993006993No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAACCCC10.6993006993006993No Hit
CGTCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
GTGCTCTTCCGATCTATTCCGGGAGACGTGTGCTCTTCCGATCTAGATGG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTTAGGGTATCGAAAACGATTAGATACCGTTGTAGTCTTAACAGTAAAC10.6993006993006993No Hit
GTGAATAGGCCCAGGCGACTGTTTAGCAAAAACACAGGTCTCTGCGAAGC10.6993006993006993No Hit
CGATAGCACTGTCTGCGATGTCGCCTGCTGATGCTGCCGACGATATGCAG10.6993006993006993No Hit
AGCAGGAACACTCCTGTGGGGGGCCTGATATTGAAGCGTGCTTCCCGCGT10.6993006993006993No Hit
GAAGAAAGAACGTCTAACTTATCCTGATCTTAAGAAAACACGTCACAACT10.6993006993006993No Hit
GGCATAGGAATGTGTTTGCCGGCGTGGCTCTCCGAGGTTGATTAAATTCG10.6993006993006993No Hit
CCTTTAAGATCGGAAGAGCACACGTCTCCCGGCAGAGATCGGAAGAGCAC10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTACACTGGGAGACGTGTGCTCTTCCGATCTACACTGGGAGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCATTCCCTTTCATAGAGCAGGTTGGAAACACTCTTTTTGTAGTATCT10.6993006993006993No Hit
TCTCTCGGGCTTGACGGCAGCTAACGCTGCCATGGTGTACCGCCCTGACA10.6993006993006993No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.6993006993006993No Hit
AAATAAAACCCTTAATTACATTCTCTAGCCAGATAGCAAAATACACAACT10.6993006993006993No Hit
AGCGTGGGGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
CCCGTGAGAGATCGGAAGAGCACACGTCTCCCTAGTCAGATCGGAAGAGC10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACTTTACACCGCCACAAATCAGGGGGTAATTTACGGGCAAACAAATGGGT10.6993006993006993No Hit
GATTTTGACTGGCTGGGTCATCTTGGGATGTTGTAGGCTGAAGTAGAATG10.6993006993006993No Hit
CTTTTGGACAACTTCAGCTTCTGCAGAAGCACCATTTGAAAGTCAGAAAT10.6993006993006993No Hit
CGTCTGCTCTGAGCACAGACCAAGGCCGCGTGCTCCAACGACAAAAGCCC10.6993006993006993No Hit
TCAAATCTAAAATAACAACTCACACCAAAAAAAGCTCTTCCAAACAACCG10.6993006993006993No Hit
TGTGAATACAGAGTGCCTCCCCATGGAAGCAAAGTGGGTGAGATTCGCCA10.6993006993006993No Hit
CGGGGGAGGTGGTTGTGGGGGGGTTTGAGCGCTTGCTGGTCGAGGGGCTG10.6993006993006993No Hit
CAGAAATCAAACAAACACATAAAGATCAAAGGACCAATAAAAAAAATATA10.6993006993006993No Hit
GTGCTCTTCCGATCTAGCTCGGGGGAGGTGGGGGGGGGGGGGGGGGCGCG10.6993006993006993No Hit
GTCTTGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
ATACACCAACCCCCGGAAATTTATCAAAAAATCACCTATAACCTGCACAA10.6993006993006993No Hit
ACACAGAACCAAGAAGGCTCAAGTGGAGAAAGGATCCATGCTTAGAGAAG10.6993006993006993No Hit
GTAACAACTAGGCAGAAGCATTCTCAGAAACTTATTTGAGATGTGTGTAC10.6993006993006993No Hit
CTGGGAGACGTGAGCTCTTCCGATCTATTCTGGGAGACGTGTGCTCTTCC10.6993006993006993No Hit
GAGTAAAACAGAAAAAAAAAAATAACCCAAAACCCGATTTGGATTTTTAT10.6993006993006993No Hit
TTCTGGGGGAGACGGGTGGTGGTGCGAGGTGGGAAGGGGGGTGGTGTTGC10.6993006993006993No Hit
GCCAACTAGAAAAGAAAAAACACACCGCCGAGAACCTATAAGACAACGAT10.6993006993006993No Hit
TAGATCTTACTATAAATATTTTAATAATTTTTTTATTATTTTATTAGAGC10.6993006993006993No Hit
GTGCTCTTCTGATCTGAGTAGGGGGAGTGCACGTCCAAGCAGTAAGGCTG10.6993006993006993No Hit
ATCGGAAGAGCACACGTCTCCCAACACAGGTCGGAAGAGCACACGTCGCC10.6993006993006993No Hit
GTTCTTATGCGGCCCGTACCCTAAACCGACACAGGTGGTCAGGTAGAGAA10.6993006993006993No Hit
CAACAAAGCAGAACTGAACACAACCCATCTACCAAGGAAAAGAGTAATAA10.6993006993006993No Hit
TCATTGATTTTCAGATCAAGAGGAAATGAATAATAGAAGCAGTTTGGGGG10.6993006993006993No Hit
GGAGAAGTGAGATAATACGATCATAAGAGGGATAATTCAGCTCATGCGAA10.6993006993006993No Hit
ATACCGAACTAACCATACACAATACAAATAAAAAAATAAAGCAAAAGAGA10.6993006993006993No Hit
GATTGGAGTGTAAGGGAATTGTATAGGATCAGTCCGGAGGGTATGGATTG10.6993006993006993No Hit
CTCACCAATATTTAGCCTTGGACGAAATTTACCGCCCGATTGGGGCTGCA10.6993006993006993No Hit
GTTCTCTTCAGCACAATTATAAATTTTTTTTTATTTTTTTTTAAATTATA10.6993006993006993No Hit
ATCTACGCGGCGCTCGCCGAAATCACCAAACAGGAGGTGCTCAACCAGTT10.6993006993006993No Hit
AATCTCGACAACTGCGAAAAATGGTACTACAGAAGGCTTTAAAAAGATTA10.6993006993006993No Hit
AAATTAAAACGTCTACGTTATGGCAAATGTGCTTTGATTAGGCTAATCGA10.6993006993006993No Hit
GCACAACACAACAATTTAATATATTAATATAAAAATTATAAAAATAAAAA10.6993006993006993No Hit
ACCGAGTCCATTGCAGCGACGAGAACAGTGCCTGTGCACGGTGGGAATGT10.6993006993006993No Hit
CGCCGGAGGTGCTGTGATGGCTGCCAAGATCCGCAAGGGCGACAAGGTGA10.6993006993006993No Hit
CTCCACTGCGGGTGGAGCACGTCGGGCTATTCAGTTAAGATTCGGCTTAG10.6993006993006993No Hit
GAGCATGGTATTTAGATTATAGACATATGTAGTCATTAGATTGGTGTTTC10.6993006993006993No Hit
GGGTGGGGGGGGGTGGGGGGTGGGGGGGGGGGGCGGGGGGGGGGGGGGGG10.6993006993006993No Hit
CATCTACCAGTGAAGATACGAATCGCACCCCAAGCCATCCGAAAAAAAGA10.6993006993006993No Hit
GTCATGGCCGCTTCCACAGCGGTCTGCGCATGGAATTCGGCCGGAAAGCT10.6993006993006993No Hit
ACCGACGACAGATCTTTCAAATGTCAGCCCTATCAACATTTGATGGTAGT10.6993006993006993No Hit
GGGAGCGATTCTCGAAGCCGAAAACGGCAGAGGCCAGGACGATCATAAGA10.6993006993006993No Hit
AGGCAGGTGCTGCCCAGTGTCCGGGGAAACGTGGTGTGGCTCTAGCAGGT10.6993006993006993No Hit
GACTAATCATTATGCAACCTTTGCACAGTAAAAATACTGCGGCCATTTAA10.6993006993006993No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGAAAA10.6993006993006993No Hit
GTCTTGGCGAGGGCGCGGCTGATCGTGACGCTGCCGTCGGCTGATTGAGC10.6993006993006993No Hit
CCACGATGCCGCCAGCAACCCGTTGCCCTTCATGCGCGCGGCCGAGCCGA10.6993006993006993No Hit
TCCTTTAACAACGAACAATTGGATGGCAAGTCAGGTTACAGCAACCGCGG10.6993006993006993No Hit
CATCAGATCATATAATAATTATTAATTTATTTATTTAATATATTAACCCG10.6993006993006993No Hit
AAATACCACTCGCCATCGGGATAGCTCGCGAGATTCCAATCAAAGGTCCG10.6993006993006993No Hit
GCATTTGGGTACCTTAAGAGAGTCATAGATACTCCCGCCGTTTACCCGCG10.6993006993006993No Hit
AAACCGGAACAGGCTAAACCGAGTATGTGTGATATCCGGCAGGAGTTGCA10.6993006993006993No Hit
CAATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTA10.6993006993006993No Hit
GTACCAGAATATCAACTGGTAACCCATCGACAACGCCTGTCGGCCGCGCC10.6993006993006993No Hit
ATTAATAGGTGCAAGGTAGTATTTCATTGTTTTGATTTGCATTTCCCCAA10.6993006993006993No Hit
AGCCTGATCCAGCAATGCCGCGTGAGTGATGAAGGCCTTAGGGTTGTAAA10.6993006993006993No Hit
GCCAACACAAAACTCCCTCAAATGACGCTCCGATCATAGAACACCGAACA10.6993006993006993No Hit
AATACGCGGGCATCAAGTCCGGTACCGAAGCAATGGATTTGCACTTTAAT10.6993006993006993No Hit
ATATTATTCCATGGTGCATATGTACCATATTTTCCTTATCCAATCCATCC10.6993006993006993No Hit
GTGACCATAAATCTAACATCTTACAAACTTTTTTTTTATTTATTTTTTTT10.6993006993006993No Hit
GAGCACACGTCTCGCAAGAGCACACGTCTCCGAAGACCACACGTCACGGA10.6993006993006993No Hit
GTAACCGACACCAAAACAAATACAAAAAGCCTATAAATCCCAGCAAACTC10.6993006993006993No Hit
ACCCAGCGGACGGCGGAGGGCACGGATTCGACGCTGGGCGAGTTCACCTG10.6993006993006993No Hit
GTCTTGGGGGGCCGACGGGGGTTTGTGGGGTGTGGGGTGGGCGCGGGTGG10.6993006993006993No Hit
GGCCCAGGCCGACGATCAGCAGCGGCAGGCGCAGCGGTGGCGGCGCGCTG10.6993006993006993No Hit
TCTCCCAGGTTAGATCGGAAGAGCACACGTCTCCCAGGTTAGATCGGAAG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATAATCCATTTAAAAAACACAAAAACAAACGAAACCATTTGCAAATCATA10.6993006993006993No Hit
CATCAGCAAAGCGTCGACGGCCTGTACTTGGCGCTTGGAGTAGATCCCCG10.6993006993006993No Hit
GATGTGAACAGGATGCCAGTTGATCTCGGCCGCCTTCTTGATCGCCTGCG10.6993006993006993No Hit
GTGCTCTTCCGATCTGCTGTGGGAGGCGTGTGCTCTTCCGATCTGCTGTG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCGTTTACTGCTCAATAACCCGTACCGCAAACCGACACAGGTAGTCAAGG10.6993006993006993No Hit
ATCTTAACCTATTCTCAAACTTTAAATGGGTAAGAACCTGAACTTTCTTG10.6993006993006993No Hit
CGTCTCCCCTTCGAGATCGGAAGAGCACACGTCTCCCAGGCCAGATCGGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACCCCCGTGTAGAACGAAAGAGAACCCGAATCACTCAATAGAACGCAAGA10.6993006993006993No Hit
CAAATACCTCGATATAAGGTACAATGATTTTGAAGGGGTTTTGCCTCCCG10.6993006993006993No Hit
CGTTAGTGGGTTCGCGCCTCCACGCCGTCGTACCGTCGCTTCACCCGGGC10.6993006993006993No Hit
GATCTACCCAGGGAGACGTGTGCTCTTCCGATCTACCCAGGGAGACGTGT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCACACGTCTCCCGACGTTGATCGGTAGAGCAAACGACTGCTCTAGCA10.6993006993006993No Hit
AGGAGACGTGTGCTCTTCCTATCTGACCCGTGCCCTGTCTCTTATACACA10.6993006993006993No Hit
TGCCACGGGAGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
CTCCAAGGCAGATCGGAAGAGCACACGTCTCCAAGGAAGATAGGAAGAGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AATCCAATTTGGCTCTGATCAACTACGTGGCTGAGCTGCTGGAAGCAAAG10.6993006993006993No Hit
CCCCCAGGGGATTCAGTACGGCAATGCATGTCAGTCCGCCAACCCCAGCG10.6993006993006993No Hit
GATCGGAAGAGCACACTTCACCCCCCAAAGATAGCAAGAAGACTCTTATA10.6993006993006993No Hit
ATTCTAGAGAAGTAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGA10.6993006993006993No Hit
AAACACCCCCGTACAAAGAAATACCACACCTAACACCACGCCAAAACAGA10.6993006993006993No Hit
CAGCAGGGTTGATCGCTGCAGTCCTGATCACCGCCCCAGCTATGGCGCGC10.6993006993006993No Hit
CTTCCGATCTTCATTGGGAGACGTGTGCTCTTCCGATCTGAATTGGGAGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTCCTTACCTGGCCTATATGCGACAGGACCGCCGCTTCAATGCCGGTGAG10.6993006993006993No Hit
ATACACATCTGACGCTGCCGACGAATAGACAGCAGTAGAACTCGGTGGGA10.6993006993006993No Hit
AACTGAGACAGCCTTAAAGAGATTCGCTCCGCCTCACGGCATCACAGCCC10.6993006993006993No Hit
CATCGAAGCCGGAAACGCCCAAAATGCCCAAGGTAACGATCCCCACAAAT10.6993006993006993No Hit
AAAGCTGCCAGTGTGGTCAAAAAGGGAAGCTCCTGTTCAAAACTAGGCCT10.6993006993006993No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA10.6993006993006993No Hit
ACCGAGGGCGACGATGCGGATGGCGAGGAGACGGCCGAAGACGGTGCCGG10.6993006993006993No Hit
GTTCAATGTCAAGCTGCAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCG10.6993006993006993No Hit
GCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGAA10.6993006993006993No Hit
GCCCCGGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGA10.6993006993006993No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTATTTTTTTTTATTGAATTTCGCG10.6993006993006993No Hit
CTACAGAACTCACCCGCAGAACATATCAACATTAAAAACCGCTACAAATA10.6993006993006993No Hit
AGATACTACAATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGA10.6993006993006993No Hit
GAGCTTTAGAGGGAGACGGGTGCTGTTGGGATCTATAGAGGGTGTCGTGG10.6993006993006993No Hit
GGGGGCGGGGGGGGGCGGGGGGGGGGCGGGGGCGGGTGGGGTGGGGGCTG10.6993006993006993No Hit
CGTCTCAGGTGCGAGATCGGAAGATCCCTGGTCTGGCGTGTGAGATAGGA10.6993006993006993No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTGGATTGGGGGGTTGGGGGGGG10.6993006993006993No Hit
GAAGAGCACACGTCTGAGATCGGAAGAGCACACGTCTCGTGGTTGAGATC10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTT10.6993006993006993No Hit
TTACGGTACCATTTTGCCTAGTTCCTTCAGCAGAGTTCTCTCAAGCGCCT10.6993006993006993No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGCGTAACGAGGAATCAGGGTT10.6993006993006993No Hit
AACGACGTGGGCAAGGGACTGCCCCTCGGGGTATATTAGGGTCCGCAGCG10.6993006993006993No Hit
ACTTTTATGGCTCTCTCAGTCGCTTCCCGGGAAACCAAAGCTTTTGGGCT10.6993006993006993No Hit
GACATGGGAGACGTGTGCTCTTCCGATCTGACATGGGAGACGTGTGCTCT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
NNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.6993006993006993No Hit
CTTCCGATCTGTGCAGGGACGTAATCAACGCATGCTGATGACATGCGCTT10.6993006993006993No Hit
CTTACAAATACGAACTTTTATTTTATATTTATATAATATTAATTTAAAAA10.6993006993006993No Hit
GGTAACGAACGTCCATCAGGTCACGACTTCGCTTGCTGCAGACGGCACCA10.6993006993006993No Hit
CTATAGTGAAACCACTCATATACCAAAAACAATACGGACACGACAGCTAT10.6993006993006993No Hit
CGTATAAGATATGAAAGGCGCTCATATCAAGGATGAGATAGGAATATAAA10.6993006993006993No Hit
TCCCCGAGTAGATCGGAAGAGCACACGTCTCCCTGACCAGATCGGAAGCT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTAAGATAACCCATATGATCAGATATTATACCGTACTAATAATAACATA10.6993006993006993No Hit
CCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAA10.6993006993006993No Hit
GATCGGAAGAGCACACACCCCGGCCCAGATCGGAAGCTGTCTCTTATACA10.6993006993006993No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers