FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16530233
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG362540.21931935260682653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT365900.026.7248556
ATCTTTT405800.023.5396027
CGATCTT515000.022.879425
CTTTTTT438050.021.4956079
TTCGGAA32850.020.6644672
TCTTTTT507150.018.718428
CGTGTGG69650.018.34549570
GATCTGG154850.017.1259216
TAGGGGT23550.016.1923734
CTTCACA83650.016.0142631
AGGGGTG55050.015.8877675
GTCCTAG14650.015.79851
ATCTGGG60850.015.5490477
TTCACAT70700.015.342642
CCGATCT1884850.015.331274
TCTAGGC13900.015.3218578
TTCCGAT1907400.015.0226632
TCCGATC1937150.014.9622743
TCACATC76100.014.8486233
CAGGGGT22400.014.8371514
Produced by FastQC (version 0.11.5)