FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119698
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTAG5450.027.6146721
CGATCTG154450.026.625885
GATCTTT140000.026.3237276
TTTCGGA14800.025.0675621
ATCTTTT143300.024.7394167
TTCCGAT504100.024.376712
TCTTTTT148000.023.8118958
CCGATCT515950.023.694744
CGATCTT190300.023.6881475
TCCGATC522700.023.6030223
CTTTTTT150750.023.3310839
CTCGTAT9600.022.96837639
ATGCTCT17100.022.7192941
ATCTGAG18700.022.6448757
GATCTGG71050.022.2156776
AATCTAG1454.6138666E-721.7241331
GATCTGA49150.021.5757626
GTATGCC11550.021.21075642
GTGTTAA4050.020.73940762
CTTCCGA600300.020.5463891
Produced by FastQC (version 0.11.5)