FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGACGTGTGCTGTTCCGATCTTGGAGGGGAGACGTGTGCTCTTCCG11.0526315789473684No Hit
GACACGGGAGACGTGTGCTCTTCCGATCTGACACGGGAGACGTGTGCTCT11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTAGTAAGCCGTCTT11.0526315789473684No Hit
TCCCTGTTGAGATCGGAAGAGCACACGTCTCCCTGTTGAGATCGGAAGAT11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACTCGCAAGCGAGATCGGAAGAGCACACCTCTCCATAGGGAGATCCCAAG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCTTAAGTACGTACGGGTGTGGCGCTTCAGCCTCTACTACGTGGTGGTCT11.0526315789473684No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATGTAGTTTTGCGTAAT11.0526315789473684No Hit
GCAGTGGGAGACGTGTGCTCTTCCGAAAATGTCTCTTATACAAATCTCCG11.0526315789473684No Hit
CCCCTACCACGACCCCTCATGCGGGCTTCCGCGACTTCGGTGCTGTGCAT11.0526315789473684No Hit
TTTCGGAAGAGTACACGTCTCCCGCCATAGATCGGAAGAGCCCACGAGAC11.0526315789473684No Hit
GAGCACACGTCTCCCTATTCAGATCGGAAGAGCACACGTCTCCCTATTCA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTATCGGAAGAGCACACGTCTATCGGAAGCTGTCTCT11.0526315789473684No Hit
CTATGTAACTGAAGCTTAGAGACTTTTCCTGGAAGCATGGTATCAGCCAC11.0526315789473684No Hit
ACCTGACACAATGTCTTTCTCGTTCTTGAAAAGGCGAGGTGCCCTTTGTT11.0526315789473684No Hit
TCCCAGCACAGATCGGAAGAGCACACGTCTCCCAGCACAGATCGGAAGAG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACTTAAACCTAAAATAATAACAACTGGCTAGATAGAATATAAAAAATGCC11.0526315789473684No Hit
CCCCGATGAAGATCGGAAGAGCACACGTCTCCCGAAGTAGATCGGAAGAG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCCTGTAACCGACCGAGCGGTCGGCCCATGAGGATCCTGAGTACGACGGC11.0526315789473684No Hit
CTTCAAGAACATATTCATATGCATAGATTTGCTGTTCAGCTCCACTAATA11.0526315789473684No Hit
CCTCGATCCAGTCCTTTTCCTCGCGAAAGGCATATGTTTTTTTCGGTGTG11.0526315789473684No Hit
GATCTCAGCAGGGAGACGTGTGCTCTTCCGATCTCAGCAGGGAGACGTGT11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAAGCCCAGGAAGGCGTTGCCGCAGCGATGAGACCTCTTTTCCAACTGCT11.0526315789473684No Hit
GTATTAGCACCCGTTTCCGGGTGTTATCCCCCTCTTATGGGCAAGTTCCT11.0526315789473684No Hit
CTTCCGATCTCGGCAGGGAGACGTGTGCTCTTCCGATCTCGCCAGGGAGA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT11.0526315789473684No Hit
GCACACGTCTCCCCTTATAGATCGGAAGAGCACCTGTCTCTTATACACAT11.0526315789473684No Hit
CTTCCGATCTGGCCGGGGTGCTCTTCCGAAAAAAAAAACTGTCTCTTATA11.0526315789473684No Hit
CTTCCGATCTCGCATGGGAGACGTGTGCTCTTCCGATCTCGCAAGGGAGA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCACGTCAGATCGGAAGAGCCCACGAGACACTGAGCGATGTCGTTTT11.0526315789473684No Hit
CTTCCGATCTAAGGAGGGGGGGACGGACTGGGAACGGTTCCTTCGGGGGC11.0526315789473684No Hit
GATCGGAAGAGCACACGTCTCCCTGTAGATCGCGGAAGCGCACGAGTCGC11.0526315789473684No Hit
TTCTGGTACACCGCAAGGCCCTGCGGCGTGCCCGTCACCGGCGAGGCGAC11.0526315789473684No Hit
CGTGTGCTCTTCCGATCTCCATTGGGAGACGTGTGCTCTTCCGATCTCCA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCTCTTCTGAAATGTTTTCCCACACGTGAGCACTTCCGATATTAAAAA11.0526315789473684No Hit
CATCATAACATAACGAAAACGAGACCTCTTCACATCACAACAGCAAAGAT11.0526315789473684No Hit
AGAAAACTCATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGTGAAA11.0526315789473684No Hit
CGTAGGCCCGGATCACCTGGGCGTTCCCCCTGGGCTCCATGCCCAGGATC11.0526315789473684No Hit
TTCTGAAACTGATGCTGCACAAGGGAGGTGCTGGTCATGAAGCATCCACG11.0526315789473684No Hit
GTTCTGCTTCAAGTCGCCGTTGGCGACCGCAGTCACCACCTTGACGATGC11.0526315789473684No Hit
CCATCGGTGTTCAGAAGCTAGTTCTTCGCTTCGCTAAGCATTCTTCGAGG11.0526315789473684No Hit
CGATCTGACAGGGGCCGAACCGATGGCGGCGCTGATCGTCTCGCTCGCGG11.0526315789473684No Hit
CTTCCGATCTGTCCAGGGAGACGTGTGCTCTTCCGCTATGTCCAGGGAGA11.0526315789473684No Hit
GAACGCCACGGCATCCCCGAAAAAATCTGGCTCGACAACGGGCGCGCCTT11.0526315789473684No Hit
CTAAAGCTCTGTTGTAAGAGAAGAAAGAGAGTGAGAGTTCAAAGTTCACA11.0526315789473684No Hit
ATTTAAAGCCTCCGCAATATCAGGCGCACTATCAATCAGTGTGCCATCTA11.0526315789473684No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC11.0526315789473684No Hit
CTTCCGATCTAACGCGGGAGACGTGTGCTCTTCCGATCTAACGCGGGAGA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATATGGGAGACGTGTGCTCTTCCGATCTGATATGGGAGACGTGTGCTCT11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTATAGACCAAATAAAATAAATTAAAACAAGAACCAAAAATTACATGAGA11.0526315789473684No Hit
GTTGCTTCTTCATGGGGTTCTCCTGCGCAATGCGCTGTGGTCCAAGATTA11.0526315789473684No Hit
ATCAAAAAGCTTAGCGCGTCGGTTAGTTATAAGTAATATCAAGTCTCCCT11.0526315789473684No Hit
TCCCAGGCGAGATCGGAAGAGCACACGTCTCCCTTGACAGATCGGAAGAG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCATAAAAATAATCAAAATCAAACTATCACACTTGTTCTGTTAATAGAAA11.0526315789473684No Hit
GTTCAAGCCAGAACTCGTGCCTGGTACGTAAATCCGCCGGGCTTAGATGA11.0526315789473684No Hit
CCTCGTACATGGGCCAAGCCTAGCCTGGAAAAGCCCAGATCTTGCGACCA11.0526315789473684No Hit
ATTATACACATCTCCGAGCCCACGAAACACAACCAATAGATGACATCCCG11.0526315789473684No Hit
TCCCGGGTCAGATCGGAAGAGCACACGTCTCCCGGGTCAGATCGGAAGAG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTAAATATCAAACAATTCAAGACCATATCACATAAAAATAAACAAACGCA11.0526315789473684No Hit
CTTCCGATCTCATACGGGAGACGTGTGCTCTTCCGATCTCGTCAGGGAGA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCCTATATAGATCGGAAGAGCACCTGTCTCTTATACACATCTCCGAGCC11.0526315789473684No Hit
GCAGGAAGACTGCTTCCATTCCAGTCGCTGTAGCGAATCCCAACGGCTTC11.0526315789473684No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATTCCGTCTTCTGCTTG11.0526315789473684TruSeq Adapter, Index 12 (96% over 26bp)
CTTCCGATCTAGCACGGGTGACGTGTGCTCTTCCGATCTCGGTCGGGAGA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAGAAAGATCGCGCCTCCGTCAACGCGGTCATGTCATTGCGTCAAGCGGT11.0526315789473684No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTTTGCCGT11.0526315789473684No Hit
GAGCACACGTCTCCCAGAGTAGATCGGAAGAGCACACGTCTCCCAGAGTA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTACGATCTTTTTTTTTTTTTTTTTTTTTTTTATATTGACAAAAACAAA11.0526315789473684No Hit
TAGTGGGAGACGTGTGCTCTTCCGATCTATAGTGGGAGACGTGTGCTCTT11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGCAAGATCAGCCTGTTATCCCCGGGGTACCTTTTATCCGTTGAGTGACG11.0526315789473684No Hit
CTTTCTTCCCTCAAACAGTTACTGAAGACCTACTATATGCCAAGCACTGT11.0526315789473684No Hit
CTTCCGATCTCAGCCGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAA11.0526315789473684No Hit
GGCCTGCTCGTGGCTCCGGCCGGTCACGACAGCGGCAAACTCCTCGCCGC11.0526315789473684No Hit
CACACGTCTCCAATCCAATAACGGAATAAAAAAAGAAAAAAAAAAAGATA11.0526315789473684No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACC11.0526315789473684No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGAAGAAACA11.0526315789473684No Hit
CGTCTGAGATCGGAAGAGCACACGTCTGAGATCGGAAGAGCACACGTCTG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCATTGGTAGTTCTAAAAAAAGAGTGTTTCAAAACTGCACTATCAAAAG11.0526315789473684No Hit
CTTCCGATCTTCTTGGGGAGACGTGTGCTCTTCCCACGCTGTCTCTTATA11.0526315789473684No Hit
GGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTAAT11.0526315789473684No Hit
CATGTATTCTATATGTAAGTTGGCGTTTCTTCCATGTCTCGAATTTTTGA11.0526315789473684No Hit
GAATGAGAGAGTGGAGTCTGGGTATGTAATAGTGAAAAATGAAAGGCCTT11.0526315789473684No Hit
CGTCTCCCTACGCAGATCGGAAGAGCACACGTCTCCCAGCGAAGATCGGA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGTAAGTTCCGACCTGCACGAATGGCGTAACGACTTCTCCACTGTCTCAA11.0526315789473684No Hit
CTCCGGTGGGAGACGTGTGCTCTTCCGATCTCCGGTGGGAGACGTGTGCT11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCCTTGAGCTGGAAGAGAGGTTTAAGGCTGCAGCCCAGCCCCTGAGCACC11.0526315789473684No Hit
CGAATTCAATTCACCGCAGACGCTGGTTGCCTTCGCGCTCGGCATCACGC11.0526315789473684No Hit
GAGCACACGTCTCCCAGTGTAGATCGGAAGAGCACACGTCTCCCTGATTA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGGCTGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAATGG11.0526315789473684No Hit
TCACAACTCTAAGGACACACGTCACAACCTCCGCGCACCCACTGCGCTAG11.0526315789473684No Hit
CATCAAATTTGCATTACGGGCTTGACTTTCTTTTCGCCTAGAAATTGCCA11.0526315789473684No Hit
CCGGGTGAGATCCCAAGACCACACGTCTCCCAAACCAGATCGGAAGCAGT11.0526315789473684No Hit
GAAGAGCACACGTCTCCCCAGTGAGATCGGAAGAGCACACGTCTCCCCAG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCTATGGTGGGAGACGTGTGCTCTTCCGATCTATGGTGGGAGACGTGT11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTCCCCGGCCCTAAAGGCCCACCCAGGGGGCACCCCCGCCTACCGGGACC11.0526315789473684No Hit
AGGGAGACGTGTGCTCTTCCGATCTAAAATGGGAGACGTGTGCTCTTCCG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC50.070.02
AACAATA50.070.066
CCAATCC50.070.010
CCAATAA50.070.015
AATCCAA50.070.012
CACACGT50.070.01
TAAACAA50.070.064
TATATCG50.070.054
ATCCAAT50.070.013
ACGTCTC50.070.04
AAACAAT50.070.065
TAAAATA50.070.049
CACGTCT50.070.03
TATCGTA50.070.056
TAAAAAA50.070.027
TAACGGA50.070.019
AAATATA50.070.051
GAAAAAA50.070.035
CTCCAAT50.070.08
GATAAAA50.070.047