FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGCCACTGAGCGAGGGCGTGTGCCGG10.78125No Hit
GAAGATCGGAAGAGCACACGTCTCCCGGTAGAGATCGGAAGAGCACACGT10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGGATGCCGGCTT10.78125No Hit
TCCTCCGGTTTATCACCGGCAGTCTTGCTAGAGTGCCTGTCTCTTATACA10.78125No Hit
GCACCATAGTGGGTGAAAGTCCCGTAGGCGAAAAGCACGGAAGCTTAGCT10.78125No Hit
CTGTTGGGAGACGTGTGCTCTTCCGATCTCTGTTGGGAGACGCTGTCTCT10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCTTGAAACCTTCAAAGTCAAAAGGATCGATAGGCCACGCTCTGTCTCTT10.78125No Hit
AATCTGGGAGACGTGTGCTCTTCCGATCTGATTAGGGACTGTCTCTTATA10.78125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACAATCGCGGGAACGTCTCCAGCGGATTGTCGACGCACATCTTCTCGA10.78125No Hit
GCACACGTCTCCCTAACGAGATCGGAAGAGCACACGTCTCCCTAACGAGA10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATAAATTACGTGATCGATCTGGAAGCTCTCCTCGTCATGAGCTAGTACCC10.78125No Hit
GAACACAACACATCTAAAAAAATATATATTAATATAAAAATTATAACAAA10.78125No Hit
GAGACAGTGTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTACCCG10.78125No Hit
GACGTGTGCTCTTCCGATCTCGATAGGGAGACGTGTGCTCTTCCGATCTC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTCCGCACCAGATCGGAAGAGCACACGTCTCC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCCTAATAAGATCGGAAGAGCACACGTCTCCCAAAGTAGAAAGGAACAGC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCGGAAGAGCACACGTCTCCCGTGTCAGATCGGAAGAGCACACGTCTC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GACCAAGGAGTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACG10.78125No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTAGG10.78125No Hit
CGCAAGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAATTG10.78125No Hit
CTCCTCGGCAGATCGGAAGAGCACACGTCTCCTCGGCAGATCGGAAGAGC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CACCTCTCCCTGCTCAGATCGGAAGAGCACACGTCTCCGTATTAGATCGG10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCGGAAGAGCACACGTCTCCCAGCTGAGATCGGAAGAGCACACGTCTC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATGAACTCAGTTTTTATTATCGACGACCACCCTGTTATACGACTTGCCA10.78125No Hit
GTCCGGGGAGACGTGTGCTCTTCCGATCTGTCCGGGGAGACGTGTGCTCA10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCATCATTGACC10.78125No Hit
ATTTGCGTCTAGGAGTCGAGTTTTGCACCAGCATTTGCTACATAGTACGT10.78125No Hit
GTGCAGCAACGACCGGTCGCGGTGGTGATGACCTCGGGTTCCGCGGTTGC10.78125No Hit
CTTCCGATCTGAACAGGGATCCTCACGTGGAATACCATTTAGAGGACCAC10.78125No Hit
TCCCAACACCCTATGTGTTCAGGTGCAGGTGCCATGACATGATTCATGGC10.78125No Hit
CTTATACACATCTCCGAGCCCACGATACGGAGCTACATCTCGAAAGCCCT10.78125No Hit
GGGAGACGTGTGCTCTTCCGATCTACGCAGGGAGACGTGTGCTCTTCCGA10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCGATCTAGTGCGGGAGACGTGTGCTCTTCCGATCTTTTTTTTTTTT10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCGATCTGGTGCGGGAGACGTGTGCTCTTCCGATCTGGTGCGGGAGA10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCTAAGTAGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAA10.78125No Hit
CTCTTCCGATCTGCAATGGGAGACGTGTGCTCTTCCGAGACGTGTGCTCT10.78125No Hit
TCTCCCACAGAGATCGGAAGAGCACACGTCTCCCATAAGCTGTCTCTTAT10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTTTCACTTCCCCGCGTTACCACCCACACCCCTATACATTCAGGGCAGGG10.78125No Hit
TAACTGAGCTGATCGCATGGTCTTTGTACCGGAGACGCATCTTTCAAGAG10.78125No Hit
TAACAGGGAGACGAGTCCTCTTCCGATCATCACAGGGAGACGAGTGCACA10.78125No Hit
ATCCCTAGTCGGCATAGTTTACGGTTAAGACTACGACGGTATCTGATCGT10.78125No Hit
CGTCTCCCGTCCCAGATCGGAAGAGCACACGTAAACCTTACCAGATAGGA10.78125No Hit
ACGTCTCCCAAGCCAGATCGGAAGAGCACACGTCTCCCAAGCCAGATCGG10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCAGGGAGACGTGTGCTCTTCCGATCTAGCAAGGGAGACGTGTGCTCT10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGATTATGCCGGCCAAATGGGTTAAACCCGGCTGCCATTTGCCTGTCTCT10.78125No Hit
TCCGATCTGCCGCGAATCCAGGAGACCGGTGCCCTTCAGCTCCTCCAGCC10.78125No Hit
GAGAAAGACAGAGACAGCAGAGAGAAACAGGCAGAAAGAGAGAGAGAGAG10.78125No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.78125No Hit
CGTATGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.78125No Hit
GCACGGGGAGACGTGTGCTCTTCCGATCTTTGTGGGGAGACGTGTGCTCC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATAAAGAC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAC10.78125No Hit
GCTGTGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAG10.78125No Hit
CACGTCTCCCTGCAGAGATCGGAAGAGCACACGTCTCCCTGCAGAGATCG10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCGAGACACGGTCCGCTCATCCTCATTCCTTCGTCATTCCTGGGCTCGCG10.78125No Hit
GCCAAAGGCCGCTTCCTGAACACGAGCACATTGCTCGCCATCACCAGCAG10.78125No Hit
AATCAGGGGAGACACACGGCGGGTTTAACGTCCGTCGTGAAAAGGGCAAC10.78125No Hit
TCCCTTGACAGATCGGAAGAGCACACGTCTCCCTGGCCAGATCGGAAGAG10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCCCTGTGCAGATCGGAAGAGCACACGTCTCCCTGTGCAGATCGGA10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGGG10.78125No Hit
CTTCCGATCTACAAGGGGCATGGTCAAGCGAATAAGCGCACACGGTGGAT10.78125No Hit
CTTGGGGGGGAGACGTATGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAA10.78125No Hit
CGTCTCCCCGTCCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTGGT10.78125No Hit
ACAATATCCAAAGTCCAAATCCTTAAATATTGAGTTTCACCAAAACCAAA10.78125No Hit
CACGTCTCCCTGGGCAGATCGGAAGAGCACACGTCTCCCAAATCAGATCG10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTGGGAGACGTGTGCTCTTCCGATCTTACTTGGGAGACGTGTGCTCTTC10.78125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTTAGGAGATCGGAAGAGCACACGGCTCCCTTACTGTCTATTATACAC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGAGACGTGTGCTCTTCCGATCTGACCCGGAGACGTGTGCTATTCCCATC10.78125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACAGGAGGGATGGCCTGAT10.78125No Hit
CCTCCAAATCGAAGCACTGCACAGGCTTGCACTCGTGTCCCCGCGCCGGT10.78125No Hit
TTCTCTACCTGACCACCTGTGTCGGTTTAGGGTACGGGCCGCATAAGAAC10.78125No Hit
CTGATCTGCCTGGCAGACGTCATGCTTGCGATGGTCGCGGTTTACGAGCC10.78125No Hit
GATCGGAAGAGCACACGTCTCCCAGACGAGATCGGAAGAGCACACGTCTC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCT10.78125No Hit
CTTCCGATCTCAGTCGGGCGCTGGAATTGCTGGTGGACGACGCCGGGGCG10.78125No Hit
GTTCGGCGCCGTGCTGGTGGCACTGTTCATCTTGCGTCCGAGCATCCTGC10.78125No Hit
CAAAGGGAGCGGCTGGTCAGCACCCCCACCAGCACCGCCCGGCCCCAGCC10.78125No Hit
CCCCGTCCAGATCGGAAGAGCACACGTCTCCCTCATAAGATCGGAAGAGC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTCCATGGGGAGACGTGTGCTCTTCCGATCTCCAGAGGCAGTCTATTAAA10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTC10.78125No Hit
GACCACACCTCTACCGATCTAAAAAGGTAGACCTCTCCTCTACCGATCTA10.78125No Hit
GCATTTGCCAAGAATGTTTTCATTAATCAAGATCGAAAGTCGGAGGTTCG10.78125No Hit
ATAAAAAACTCCTAGCCAACGATACTATCGTTACAAAGGTATGGCGTATA10.78125No Hit
CTATGATTATGATGATGGCTGTGGTTGTTGATCACTGGCGTGCTTTTGCA10.78125No Hit
ATCTCATTGCATGATTGATCACAAGCATCCACGCCCTTCGATTGAAACTT10.78125No Hit
GCCTTCACTTCTGCGTCCATGAAGCCGAACGAGGTGCCGAAGATCAGCTT10.78125No Hit
GATCGGAAGAGCACACGTCTCCCTTGTAAGATCGGAAGAGCACACGTCTC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCA10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGATAATGCTGGTTTCGAGCATCATCGTGGTGATCCTGCTTCCACCCCAC10.78125No Hit
GACTGGGGAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCT10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA10.78125No Hit
TCCGATCTCTGTTGAGACGTGTGCTCTTCCGATCTCTGCTGGGAGACGCT10.78125TruSeq Adapter, Index 2 (95% over 24bp)
CCCCCGACAAGATCGGAAGAGCACACGTCTCCCGCGCCAGATCGGAAGAG10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGGTTCGA10.78125No Hit
TTCTACGACAGCCTGCTCGGCAAGCTGTTCGTGCTCGACAAGGATCGGCC10.78125No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATATCGTATGCCGT10.78125No Hit
GTACTAACAACGATGCTAAGAAGAAATTAGTTGAAATTACTGATAAAGCA10.78125No Hit
TTATTAGGGAGACGTGTGCTCTTCCGATCTTATTAGGGAGACGTGTGCTC10.78125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACAAAACTTATCCCAAGCTAGATATTAATAACACAATTATAAATAGAAA10.78125No Hit
TATCGGAAGAGCACACGTCTAGGAAGGGCTCTCGTAGCGCAAGATCTGAC10.78125No Hit
GGAGACGTGTGCTCTTCCGATCTCGCTTGGGAGACGTGTGCTCTTCCGAT10.78125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCAACCGTTCGCGTCAAAGTCGCGCAAAAACAAATAAATGCGAGTATAG10.78125No Hit
GTGCGGCTTCTCCTCCTCAGGAACGATCTCACCAACCTCCAGGCCGTTCT10.78125No Hit
GGGGCAGATCGGAAGAGCACACGTCTCCGGGGCAGATCGGAAGAGCACAC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCGGAAGAGCACACGTCTCCCACTGCAGATCGGAAGAGCACACGTCTC10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTGAACAGGGAGACGTGTGCTCTTCCGATCTGACCAGGTAGA10.78125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGAGCCCCCATTGAGATCGGAAGAGCACCTGTCTCTTATACACATCTC10.78125No Hit
TTTCCAGGATGCGCGCATGGGTGCGCAGCAGCATGTCGTCGTCGACCAGG10.78125No Hit
GAAGAGCACACGTCTCCCAACACAGATCGGAAGAGCACACGTCTCCCAAG10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAAAATGCCTGTCTGTAAATTCTCTCATATTTTTCAAGAGTGTATCAGGC10.78125No Hit
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC10.78125No Hit
GATCTTAACAGGGAGACGTGTGCTCTTCCGATCTCTAGACGTGTGCCTGT10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCTAAGATCGGAAGAGCACACGTCTCAAAGAACATATCAAAAGCCCACCA10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CTTCCGATCTGCATTGGGAGACGTGTGCTCTTCCGATCTGCATTGGGAGA10.78125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACGAGACGTGTGCTCTTCCGATCTAAGTTGGGAGAGTGATTTGTCTGGT10.78125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGTAAAGTGGCGCACAGGTGAGTAACACGTGACTAACCTACCCTTGAGT10.78125No Hit
TCCCATTACAGATCGGAAGAGCACACGACTCCCATTATAGATCGGAAGAG10.78125Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCCTAAACCGACACTGGTGGATAGGTAGAGTATACCGAGGCGATCGAGTG10.78125No Hit
GAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGA10.78125No Hit
CGTCTCCCCCGTCCAGATCGGAAGAGCCCACGAGACACTGAGCGAGCTAG10.78125No Hit
TCCGATCTCTGACCATCCTGCCCGAGACCCCGAAAGACGCCCAGGCGATC10.78125No Hit
TCTGACACCAGATGTGTGGGGATTTCTCCTCACCACTAAGCAAGAAATCA10.78125No Hit
CGTCTCCCCGTCCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGT10.78125No Hit
CATTGGAAGCGGTCACGCAGGAATACGTTGAAACGCGTGAAGGCCGGATA10.78125No Hit
ATATTAGGCCATGAGATTCCAGATTTTTTAGTTCCACTTTCAAATCAGGC10.78125No Hit
GCATGTAAGATTTCCACTGAAATATCTGCTTCCAGATGTATTAGAGTTCC10.78125No Hit
AGACCAGCCTGGCCAAAATAGTGAAATCCCATCTCTACTAAAAATACAAA10.78125No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers