FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGCGTCAGATGTGTATAAGAGACAGACTTAGAGTGCCCAACTGAATG31.948051948051948No Hit
ATCTTATACGGGCGCTTGATCACCTACCCAAAATCCACGGAATTCCGACG10.6493506493506493No Hit
GCCTTCTGCCGAGGGTGCAGGGAGTCAGAAAGTCTTGTGTTAGTGGAAGG10.6493506493506493No Hit
GCCCATCAAAAATGAGCAGCCCGCCGTGGTCGAGTTCCGTGAGGTGACCA10.6493506493506493No Hit
CACGTCTCCCTTTGAAGATCGGAAGAGCACACGTCTCCCTTTGAAGATCG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTGGGCAGGGAGACGTGTGCTCTTCCGATCTGTGTAGGGAGA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCATTCGAGATCGGAAGAGCACACGTCTCCCAAGGCAGATCGGAAGAG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTATAATCCAGTCCCATAATATATGTACATTTTCTTCAGAGAATTGTAAG10.6493506493506493No Hit
AAAGTACCCCGGGAGGGGAGTGAAAGAGTTCCTGAAACCGGTCGCATGCA10.6493506493506493No Hit
GTCCTCGCCATGACAGGTGGCGGACACGTGGTTCTCGCCGTCATAGCCAT10.6493506493506493No Hit
CACATAACCCAGACGATTGCACATCCCATCTAAACAGCCCTGTCACATAT10.6493506493506493No Hit
GAAGAGCACCCGTCTCCCTGTGCAGATCGGAAGAGCACACGTCTCCCTCT10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCCCAGCGGAGATCGGAAGAGCACACGTCTCCCAGCGGAGATCGGAAG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGTGCTGCAGGGCACAGTCGATGGCGGCCGCGGTTTCTTCCGGCCGCT10.6493506493506493No Hit
CGTCTCCTCGTCAGATCGGAAGAGCCCACGAGACTGCAGCTAATCGGGAA10.6493506493506493No Hit
ATACAACAAATTCACCGATGTCATCTAAATACACCAGCACCCGACTATAC10.6493506493506493No Hit
CACGTCTCCCAGACGAGATCGGAAGAGCACACGTCTCCCAGACGAGATCG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CACCCGGATCGCGCTGATCATGGTGGCGGTGGCCCTGAGCGCCCTGACCG10.6493506493506493No Hit
GATCTGGACCGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAA10.6493506493506493No Hit
CTGGAAACCTTAGTCAATCGGTGGACGGGATTCTCACCCGTCTTTCGCTA10.6493506493506493No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGGATGCCGT10.6493506493506493No Hit
CTTCCGATCTAGTCTGGGGGTTCGTTTTTTCAACGATTAAAGCTGTCTCT10.6493506493506493No Hit
ATTCCGATCTATCTAGGGAGACGTGTGCTCTTCCGATCTATCTAGGGAGA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGTATATGGAAACGGAGCTGAGCATTAGAGTTTGGATCATGATCGTGA10.6493506493506493No Hit
ACCACAACCTGCGCGCGCTTTATGCGAAGGTACCGGTTTTGGCGCAATGG10.6493506493506493No Hit
CCTCAAGAGATAGGAAGAGCACACGTCTCCCTTGACAGATCGGAAGAGCA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCACACGTATCAACCACAAGAACTCAAGACCAAACCACTCCAGGCACAGA10.6493506493506493No Hit
GTGCTCTTCCGATCTGGGATGGGAGACGTGTGCTCTTCCGATCTGGGATG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGATTGGCACATACAAATTGATCCGTAGATTTCCGAATGGGGCAACCCG10.6493506493506493No Hit
TGGGAGACGTGTGCTCTTCCGATCTGTTATGGGTGACGTGTGCTATTCCG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGATACTGGCAAGGGTGGTCAGCGTGAATTCACCGGTCTGGGCAACTG10.6493506493506493No Hit
GTTCAGCACCACCACGTGTTAGCAGACATTGCCGATGAAGTGCATGTCGT10.6493506493506493No Hit
GCCGAGGGCGCGACCGCGATGGTGCCCGGTAGATCGGAAGAGCACACGTC10.6493506493506493No Hit
GTGCTCTTCCGATCTTTGTAGGGAGACGTGTGCTCTTCCGATCTTTGTAG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGAAGGCTCGTCCGCTCTCGGTTAGTCGGGTCATAAGCTGAGGCCGAC10.6493506493506493No Hit
CGTCTCCCCTTTTAGATCGGAAGAGCACACGTCTCCCCTTTTAGATCGGA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCCTACTTGTCGTACACCTAGTTCCACAAAAGTCATTTTTCATACGGGGC10.6493506493506493No Hit
GTGCCTCCCTGCTCCGAGGGGTTCCCCCCAAGGCCCAGTGAGCCCGACCC10.6493506493506493No Hit
CACGTCTCCCAGACTAGATCGGAAGAGCACACGTCTCCCGTGGGAGATCG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC10.6493506493506493No Hit
TAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATG10.6493506493506493No Hit
ACTCTCACCCACTCCGGTACGCCTTCCCAGACGCTTCACTTTCACGCAAC10.6493506493506493No Hit
CTTCCAAATGTGTGCATATTGCAGCAAACTTGTTAGTTTCGATTTTCGGA10.6493506493506493No Hit
GATCTAGAGAGGGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTC10.6493506493506493No Hit
AAATAACTTTGACTACTCTCTTCAACATAAAAACCCTACCACAAAACCAA10.6493506493506493No Hit
ATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTC10.6493506493506493No Hit
GCCCAGAGTAGATCGGAAGAGCACACGTCTCCCTGAGCAGATCGGAAGAG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCCCAACTTTCAATTTCATTGCGCGCTCGGGTTTTCCACCCAAACACT10.6493506493506493No Hit
GATCGGAAGAGCACACGTCTCAAGATCTGAAGAGCACACCTCTCCCTTTG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ACCGTGGGAGACGTGTGCTCTTCCGATCTAGCAAGGGCTGTCTCTTATAC10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTTCTTTGGGAGACGTGTGCTCTTCCGATCTTCTTTGGGAGACGTGTG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTATAGAGTGTCCTACAACCCCAACAAGCAAGCTCCTTGGTTTGGGCTCT10.6493506493506493No Hit
CTCTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTC10.6493506493506493No Hit
GATAGGGAATGTTCAACTCTGTGTCCTGAATACAAACATCACAAAGATGT10.6493506493506493No Hit
ATTTAGAATATTTCAATTAACATATAAAAACTTATACCTAAATTATTACT10.6493506493506493No Hit
CTTCCGATCTGGGCTGGGCAAATGTCTGCCCTATCAACTTTTGATGGTAG10.6493506493506493No Hit
GGCCAGTCACCTCGCGCTGATCTCGCAGCCCGACCCGATCGCGCGGCTGA10.6493506493506493No Hit
CGTCTGATCGGAAGAGCACACGTCTGATCGGAAGAGCACACGTCTGATCG10.6493506493506493Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CTTCCGATCTGCACTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6493506493506493No Hit
CGTGTGCTCTTCCGATCTCGGGTGGGAGACGTGTGCTCTTCCGATCTCGG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCAAGCGCCATGGCCTATCCCAACTGCCCAGTGAAGCCAAGGATGCGC10.6493506493506493No Hit
GAGATACCCTTGCTCGCAAGCGCCTCAAGCTCAGCAACGAGTGACTGCAC10.6493506493506493No Hit
AAAGTGATCTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA10.6493506493506493No Hit
GTCTAAGGGTTCCTGATCAACGTTAATCGGATCAGGGTCTGTCTCTTATA10.6493506493506493No Hit
CTTCCGATCTCCCACGGGAGACGTGTGCTCTTCCGATCTCCCACGGGAGA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACATAATAGAGGCGATGGCTGGTTGGCTGCATGGCGAAGGCTCCAGCGCA10.6493506493506493No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAGATCACACCA10.6493506493506493No Hit
GGGCAAGGAGAAGTACCAGACGTCCGTCAAGGACAAGGCCGAGACGAGCG10.6493506493506493No Hit
ACCAGGATAAAACCTAATATACAACCAGACTACAGCTAATCTCGAAATCA10.6493506493506493No Hit
TCTACAGCCCGCGCCCGGGCGTGATCTATAAGTCGCTGAAATTACTCGAA10.6493506493506493No Hit
CACCCCGGTTGAGATCGGAAGAGCCCACGAGACCGGAGCCTATATCCTAT10.6493506493506493No Hit
GTCTGTAACTGACGCTGATGTGCGAAAGCGTGGGGATCAAACCTGTCTCT10.6493506493506493No Hit
GAGCACACGTCTCCCGGCATAGATCGGAAGAGCACACGTCTCCCGGCATA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTGCCCAAGCGACTGTTTATCAAAAACACAGGTCTCTACTAA10.6493506493506493No Hit
TTACAGACCAGAAAGTCGCCTTCGCCACTGGTGTTCCTCCATATCTCTGC10.6493506493506493No Hit
CTATAAAGGGAGACGTGTGCTCTTACGATCTACAAAGGGAGACTTTTGCT10.6493506493506493No Hit
ACCCTGGTCCAGTATCCAGTATTCTCAGCGAAAAATAACTTAAGAATGCA10.6493506493506493No Hit
TCTAAGGTGAGCGAGCGAACTCTCGTTAAGGAACTCGGCAAAAATGACCC10.6493506493506493No Hit
CGACACCGGTTGTTCCACCACCAATAGTTACCTTTCGATTTGTTTATCAC10.6493506493506493No Hit
GATAACAGGCTTATCTCCCCCAAGAGTTCACATAGACGGGGAGGTTTGGC10.6493506493506493No Hit
CGCCTGGGAGACGTGTGCTCTTCCGATATCGCCTGGGAGACGTGTTCAAT10.6493506493506493No Hit
TCTCTGGACGACCCATTGCTGCCTACGATCCCGAGGGCTTGATCTTCGCC10.6493506493506493No Hit
GATCTCTACAGGGAGACGTGTGCTCTTCCGATCTCTACAGGGAGACGTGT10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC10.6493506493506493No Hit
GAATGGGAGCGCATAGCCAAGCTCTGCGACTTCAATCCCAAGGTGAACAA10.6493506493506493No Hit
GCTCTTGACGGCGGCGGGCGCGGATCATGGCGCGCGCAAACTGCGCCGCC10.6493506493506493No Hit
CGACAGCAGGAGCAGCTGCAGCCGGAGCGGGAGCAGCAGCAGCCGGGGCT10.6493506493506493No Hit
GAAGAGCACACGTCTCCCTGGGCAGATCGGAAGAGCACACGTCTCCCTGG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAAGAGCACACCTATCGCTTTTGAGATCTGAACAGCACAAATATAACTAT10.6493506493506493No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC10.6493506493506493No Hit
CCTTAGATATCGTAGCCGTTAATCAGTATCCATCTCCGAAAAAGAACCAA10.6493506493506493No Hit
TCCCACCACAGATCGGAAGAGCACACGTCTCCCACCACCTGTCTCTTATA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTCACACTCTTTGTCGTTACTCATGTCAGCATTAGCACTTCTCATACCTC10.6493506493506493No Hit
AAAGCCTACCCTATCCCTATTTGGGAATCAGGGTGCCTCGACACAGCAAC10.6493506493506493No Hit
ATACGAAAGTGGGTATTCTCGGCATTATCGGTGGCAGTTAAGTTGCGGAT10.6493506493506493No Hit
TAATTTTTATATTAATATATTTATTTTTATACGTGAGCACAACCGAACTA10.6493506493506493No Hit
TCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATACCGAAAAC10.6493506493506493No Hit
GTCCGTGACCAGCTGCTCGTCTGGGCCGAACACACACTCGACAGCCTCGA10.6493506493506493No Hit
CCACCGATCCGCGCGCTCCGCCGGAGTGGTTCGTCGCGCTGGTTCATCCC10.6493506493506493No Hit
GAGCACACGTCTCCCAAGTGAGATCGGAAGAGCACACGTCTCCCAAGTGA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAAGGGCCATCAGAGAGGGTGAGAATCCCGTCTGGGACGGGGTGTCCGC10.6493506493506493No Hit
GTTCGGTCCCTATCCGTCGCGGGCGTTGGAGATTTGAAGAGAGCTATCCT10.6493506493506493No Hit
CCTTAAGAGTGCCGGATTTGCCTAACACTCAGTCTTACTGCTGTCTCTTA10.6493506493506493No Hit
CACCCAGCCCGCAGACGAACACAAGAGCGCCCAAGTAGGCGCTGAGCAAG10.6493506493506493No Hit
GTGTCGCCAAGCTTCCCAGCTTGTTCCTCTGTCTCTATCGCTATCACTAA10.6493506493506493No Hit
CTGAAAAGGGAGACGTGTGCTCTTCCGATCTGAAAAGGGAGACGTGTGCT10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCCCTTGGCAGGCTGCGGCCGTCCGGCGGGCGTTTAACGACCAACTTAGA10.6493506493506493No Hit
TCTCCAAGTACACCAACCTGCCGCCCGATCGCGCTGCGGTGGACTGCGCC10.6493506493506493No Hit
ATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGAACTTGCGTTGGG10.6493506493506493No Hit
CTCGACGGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6493506493506493No Hit
GTCCACACACTTAGCCCCGGACAACCATCACCGGGGATGAGCTACCTCGC10.6493506493506493No Hit
GCTTAAAAGCAGGCTTTCAAATGCCTGAATATTCTGTGCATGGGCTGTCT10.6493506493506493No Hit
CTTCCGATCTAGACAGGGAAAGATCGGAAGGCAAAAAAAAAAAAAAAAAA10.6493506493506493No Hit
TTCTTGGCGCCTGCTCCCACTTCATGGTTGAGCAGCCTGGATAGGTCGTA10.6493506493506493No Hit
AGCATGCTGATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCGAGTT10.6493506493506493No Hit
GTGCTCTTCCGATCTCGACAGGGAGACGTGTGCTCTTCCGATCTCGCCAG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGCCCGCATCCTGCAGGGCCTCGGCGGCGCGATGATGACGCCGGTTGGGC10.6493506493506493No Hit
GCCGTGGACGATGATGCGCTCGCCGATCGGCTCGCCGGTGTGCTGGGTGG10.6493506493506493No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGATGCG10.6493506493506493No Hit
ATTCACACTCGGACACCTGTGTCGGTTTGCGGTACGGGTCACTGTGTTTC10.6493506493506493No Hit
CTTAAGAAAAAAAAAAAAAAAAAAAAAAAAGAAAGATAGTTGGAGCAACC10.6493506493506493No Hit
CGCTAGGGCGTTGCTTTTTGATCCTTTGATGTCGGCTCTTCCTATCACCC10.6493506493506493No Hit
CAAACACAGTTCCCATTAAACTTCCAGCACCGCGGAAGCACACAGTACGT10.6493506493506493No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTC10.6493506493506493No Hit
GTCTCTCGCGCTCATCCGTGAGCCGGCAACGGCGGATGCGGGATCGCCGT10.6493506493506493No Hit
GTGCTCTTCCGATCTCACCCGGGACTGTCTCTTATACACATCTCCGAGCC10.6493506493506493No Hit
AGGGAGACGTGTGCTCTTCCGATCTTGCTAGGGAGACGTGTGCTCTTCCG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCTATAAAGATCGGAAGAGCACACGTCTCACGTCAGATATCAAACTCT10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CGTTACGTGGCCGGAGGAGCGAGATCCCCACTACGTGGAAACTCTGATCT10.6493506493506493No Hit
ATCCGAGGCGCCGAAGTCAACCGTTTTCGCCTTGATCTGCTTGATGCCAG10.6493506493506493No Hit
TGCATCTGCAGCGCACTTCATGGAATCAGGTATCGATTGCTGCATTAGCT10.6493506493506493No Hit
CTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGAT10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATAATAGAATAGGACGTGCGGTTCTATTTTGTTGGTTTCTAGGACCGC10.6493506493506493No Hit
TCTCCCACGTTAGATCGGAAGAGCACACGTCTCCCGTGTGAGATCGGAAG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACCCGGGGAGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGA10.6493506493506493No Hit
GTATTGGAAACTGGGAAACTTGAGTGCAGAAGAGGAAAGTGAAATTCCAT10.6493506493506493No Hit
GGCTGAGGGTGGCCAGCGGCACCTTCAAATCGCGTCCCGCCGCAGATAGG10.6493506493506493No Hit
GTCTCCCGGAGAAGATCGGAAGAGCACACGTCTCCGGGCGAGATCGGAAG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTTTCACATCAGACTTAAAAAAACCGCCTACGCGCGCTTTACGCCCAAT10.6493506493506493No Hit
GAGTAGGGAGACGTGTGCTCTTCCGATCTGAGTAGGGAGACGTGTGCTCT10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTAGCGGCGGAGGCGGCGCTCGGCCCGTTCGACGAGCCGAAACGGCTGCG10.6493506493506493No Hit
TAATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGTTGCCCGC10.6493506493506493No Hit
GAAGAGCACACGTCTCCATGGAAGATCGGAAGAGCACACGTCTCCATGGA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCTGGGGAGGCCTCAGGAAATTTACAATCATGGCGGAAGACAAAAGTA10.6493506493506493No Hit
GTTCAGATCGGAAGAGCACACGTCTCCCACCTGAGATCGGAAGAGCACAC10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCCCTTGACAGATCGGAAGAGCACACGTCTCCCTTG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGACAAATTGCGTCTAGCAATAATGAGATTGAGCAATAACAGGTCTGTGA10.6493506493506493No Hit
GATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGAC10.6493506493506493No Hit
TCCTTGAGTAGAGCGGGGCACGTGAAATCCTGTTTGAATATGGGGGGACC10.6493506493506493No Hit
TCCCGTCTAAGATCGGAAGAGCACACGTCTCCATGGCAGATCGGAAGAGC10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTCCCGTGCAAGATCGGAAGAGCACACGTCTCCCATTAGAGATCGGAAGA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGGCTGGACGGCTCAGGCCTATGCTGGGCTGCCCTGCGACCACCGAGCCC10.6493506493506493No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers