FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2531371
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC70830.27980884666846545No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC61880.24445251209719948No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC53730.21225652028090705Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC48450.19139825809808203No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA46740.18464302545932618Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC43030.16998693593313663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA6700.031.8666061
TTCGGAA9600.023.3335552
ATCTTTT49050.022.7613567
TCTTTTT51150.021.3479148
CTTTTTT52700.020.7192159
CGTGTGG37050.020.50033270
GATCTTT61300.020.0409766
GACTCGA119650.018.6344370
GACGATA3650.018.21935368
ACGATAG4050.018.14868269
CTATTAG1401.8663771E-417.5005131
AGGGGTG18250.017.2604395
AGACGAT8600.017.09318767
GTATTGG6150.017.0736711
AGACTCG132700.016.90705169
TCTAGGG9750.016.8719582
GAGACGA16000.016.84357866
GACGTTT9600.016.77099268
GTATAGC2301.2089185E-716.7396221
GGGGTGC29950.016.7113466
Produced by FastQC (version 0.11.5)