FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTTAACGAGAGTTCACTCGCTCACCTTAGAATACTCATCTCGACGA22.564102564102564No Hit
GAATCGAACGAGCCGAACGCGCCATTCGGCTGTCCCCGCTGGATCAACAA11.282051282051282No Hit
GGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCC11.282051282051282No Hit
GTTTAACTTGAGTGCAAGAGGGGAGAGTGGAATTCCATGTGTAGCGGTGA11.282051282051282No Hit
GCAGAGCCCAGGCCCAAGCGACTGTTTACTAAAAACACAGGTCCGTGCCA11.282051282051282No Hit
CCCCTAACAAACAAAAGATCAACTACCAACGTATAAACCCCAACAACTTA11.282051282051282No Hit
TAAAAATAAATTATATATATTAATATAAAATGTCTCTTAAACACATCTCC11.282051282051282No Hit
GTCCCTACGCTGCATTCGCTTGCGATACTTTTGCAGCTGCCTCTCCCGAT11.282051282051282No Hit
ACCCATGAGGATCCTGAGTACGACGGCACACGTGATATGCCGTCGGAAGC11.282051282051282No Hit
CCCCTCGCAAGATCGGAAGAGCACACGTCTCCCAGCGGAGATCGGAAGAG11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGATGAGCGCATCGCGCTCGAACAGGCAGTAGCCCTCGGGATCGAACATA11.282051282051282No Hit
ATTTCAAACTGGGGCGGCTGCGAGAAGCGACGCGTGTTCGTTTCCAAATG11.282051282051282No Hit
AATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCATGTATTAGC11.282051282051282No Hit
CCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTA11.282051282051282No Hit
CCAACGAATAATAGAATACGACCTCTCGTACAATTTTCAAGCTATCTAGC11.282051282051282No Hit
ATTGTGCAGCGCGATCTTCCCAAGGACCAGTGGACAACGCCTGAAAATGA11.282051282051282No Hit
CTGGGGGGAGACGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
GCCAACATCCTAGTTGTCTGGGCAACGCCACATCCTTTTCCACTTAACAT11.282051282051282No Hit
CAAATACACATCTCCGAGCCCACGAGACTAGAGCAGATGTCGTAAACCCT11.282051282051282No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGGCGGCCGC11.282051282051282No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGCGT11.282051282051282No Hit
TCACTATTGTTTCCTTCTCCTACGGGTACTGAGATGTTTCACTTCCCCGC11.282051282051282No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAGA11.282051282051282No Hit
GGCGCACGCATTCTTCGTTGATGACAGGCAGCATGCCGGGCATGGCGGCA11.282051282051282No Hit
CTTCCGATCTAGGCGGGGGAGACGTGTGCTCTTCCGATCTCGTCCGGGAG11.282051282051282Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTCCCGGGCATGATCGGATGAGCACCCCTCTC11.282051282051282No Hit
CGTGTGCTCTTCCGATCTAACCGGGGGTGCTCCTGTCTCTTATACACATC11.282051282051282No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTAAGAC11.282051282051282No Hit
ACACATAAGATCTTCACCGGGACACCAGTGCACTACAGAACTTAACCCCA11.282051282051282No Hit
ATCTTCACCACGTCGCTACCGCCGGCGGTCGCTGCTGGCGCGCTTACCAG11.282051282051282No Hit
GCATGGTGGCCACCGTGTTCTCGATAAGCGGAGCCGCATTCGTGGTGACC11.282051282051282No Hit
GTGCTCTTCCGATCTTTACAGGGAGACGTGTGCTCTTCCGATCTCAACTG11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GACCCAAACTTCCGCAGCAACCAGCTACTAGAACGTTCGATTAGTCTTAA11.282051282051282No Hit
AACCACACTCATATTGAGGTCAGCATTACTGTGATACCTGAACTTTAAGC11.282051282051282No Hit
ATACACAACTCAGAGCAAACGAGACTGCAGCTAATATCTTATGCCGTCAT11.282051282051282No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG11.282051282051282No Hit
CGGATGGTCATGGTAATGTAATTGGTACAGGTACAATCACCGGTAATAAC11.282051282051282No Hit
AGACCAGGGCAGTTGGTGTCCTTCTCTTCCTTGCCGTAGATGCACATCCC11.282051282051282No Hit
CGGAAATAGCGGTCTAGCGGGCCTTTCTGCCTCTCTGTGTGCTTTTGAAA11.282051282051282No Hit
GAAGAGCACACGTCTCCCAAGGGAGATCGGAAGAGCACACGTCTCCCAAG11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAATACTAGGCGGCAATACCCAGGCCACCTCAGGAATGTCCCGCCGTGAG11.282051282051282No Hit
CTTCCGATCTGTGCTGGGCTGCTCCCCGTTCGCTCGCCGGTACTAGGGGA11.282051282051282No Hit
CGGCCAAAGCGGTTTTGACGGCCGATGCGAGTAGGCGTCGCGGCAGGCCT11.282051282051282No Hit
GTGGCGCAGACCCTGGTAGCAGCCGATCTCGATCTTGCGACGGATATCGG11.282051282051282No Hit
ATACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC11.282051282051282No Hit
ACAAAGGGAGACGTGCGCTCTTCCGATCTGCGGTGGGAGACGTGTGCTCT11.282051282051282No Hit
GTTTGGGAGACGTGTGCTCTTCCGATCTCGTTTGGGAGACGTGTGCTCTT11.282051282051282Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCGGCACTGATCTCCATCATGGCGTTGTCATAGCCTTCGCGATCGAC11.282051282051282No Hit
TTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCA11.282051282051282No Hit
AATAAAGCCAGTCGTAGTCCGGATCGCAGTCTGCAACTCGACAGCGTGAA11.282051282051282No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA11.282051282051282No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGACTAGA11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCGGAAGAGCCCACGAGACTAGCGCTCATCTCGTTTGCCGTCTTCTGG11.282051282051282Illumina PCR Primer Index 7 (95% over 22bp)
GATGCGGACCATGCGGTTGCGCTCGTCCTCGCTCGACATCAAAAACGGCA11.282051282051282No Hit
ATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTAT11.282051282051282No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT11.282051282051282No Hit
GTCCGAGATCCTGCGCGCCGAAGGCGCCAAGCAGGGCCAGATCCTTCAGG11.282051282051282No Hit
GTCCTCCTCCGAGGCAAACCAACCCTCGAATACATGCCCGTGGCGGCACC11.282051282051282No Hit
GGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGG11.282051282051282No Hit
GCGTCACGGCATGGCCGCGCTCGGCCAGTGTCGTCGCGGCAGCCAGGCCG11.282051282051282No Hit
CTTCCGATCTTTTAATTTTTTATTTTTAATTATTAAATAGACAACCCCTA11.282051282051282No Hit
CACTCGTTTAAGAGCACTAATGCAGGTTTTTAAATAGGAGGAACATATGA11.282051282051282No Hit
CCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGTAAATTAC11.282051282051282No Hit
TCCCACCACAGATCGGAAGAGCACACGTCTCCCACCACAGATCGGAAGAG11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCGGAAGAGCACACGTATCCCTGACAAGATCGGAAGAGCACACGTCTC11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTGGCTGGGCGTACGGCGCGGGCTGCGGCCGCTACTGCAGTTTAGTG11.282051282051282No Hit
AACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTGTGCT11.282051282051282No Hit
AGATCGGAAGAGCACACGTCTCCCTCTGTCTCTTATACACATCTCCGAGC11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAACACAGCCTATGCACCAGGGCCCCCCCAATACATCGGAAGCTGTCTCT11.282051282051282No Hit
CCTGAATCTAACATAATAAAAATAATAATGTTGGTTTCTTTTTGTCTGTT11.282051282051282No Hit
ATGCAGGCCAACGTGCTTGATCGCCAATCTCCAGGCAACGGCAGAATGCC11.282051282051282No Hit
GAGAGAGAGAGAGAGAGAGAGAGATGAGAGAGAAGAGAGAGAAGAGAGAG11.282051282051282No Hit
CTTCACAGACGTGATATCTTAAGAGAAGTGTTCTATTAAGACACCAATGA11.282051282051282No Hit
TTCCTATAGCTTCTGCAAGCACCATATCCTCTTGGTAGGTTATCTTTATG11.282051282051282No Hit
ATGCTCTTCCGATCTGTGACGGGGTGTGCTCTTCCGAAAAAAAAAAAAAA11.282051282051282No Hit
GAGTAACGACTTCCCCGCTGTCTCAACCATGAACTCGGCGAAATTGCATT11.282051282051282No Hit
ATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGCTGTCTCTTATAC11.282051282051282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTC50.070.040
TTGTCTG50.070.042
TGGTTTC50.070.032
ATCCCAT50.070.057
TGTTGGT50.070.029
TCCCATA50.070.058
AATCTAA50.070.05
TAGTGAC50.070.063
TTTCTTT50.070.035
ACTGAAT50.070.070
AATCATC50.070.053
TGTCTGT50.070.043
TAAAAAT50.070.017
ATCTAAC50.070.06
CATCCCA50.070.056
TGTTTTA50.070.047
TTTTTGT50.070.039
AAATAAT50.070.020
GTTTCTT50.070.034
TGACACT50.070.066