FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633815
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC20890.12786025345586863No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA20830.12749301481501885Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20800.12730939549459394No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC20470.1252895829699201Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC19660.12033186131844792No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC19550.11965859047688998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT115800.029.436017
TCTTTTT116100.029.3298038
GATCTTT120100.028.8527476
GTCATGT5300.028.3971268
CTTTTTT121100.028.147739
TAGGGGT8350.028.0864334
CATACGT2200.027.0479624
CAGGGGT10400.026.9255734
ATGCTCT11550.026.3668861
TTTCGGA4600.025.1117921
AGGGGTG28750.024.2283345
TTATACG1902.1827873E-1123.9495894
AGCATAC2350.023.8319972
CGATCTT169300.023.3423865
AACGTGT3150.023.3362121
TCATGTC4500.023.33406869
CGTGTGG24550.023.23902170
GGGGTGC52400.022.3773446
GTTACGA2700.022.03570764
CTGGGGT8200.021.7703114
Produced by FastQC (version 0.11.5)