FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3580337
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC72180.2016011341949096No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC60400.1686992034548703No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC56310.15727569779045938Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA53760.15015346320751372Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC51270.14319881061475498No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC46640.13026706703866145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA11800.027.880961
TTCGGAA12900.025.774832
CGTGTGG64250.021.57348870
TCTTTTT65600.021.1262728
CTTTTTT66050.021.0880179
ATCTTTT67350.020.7851877
CTAATCT22450.020.73641270
GACTGCA148600.020.70467270
CATACGT4000.020.1244344
AGCATAC4050.019.876262
GCTAATC27200.019.81755669
GTATAGT2705.456968E-1219.444171
GCTAATG7400.019.39324270
GATCTTT81300.019.1569026
TAATCTC13650.018.97568169
GAGCATA5100.018.5291481
AGACTGC168550.018.39938469
ACGTTTG9650.018.13597969
TTACACC5900.017.796114
TAGGGGT7850.017.3880464
Produced by FastQC (version 0.11.5)