FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755677
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT46100.025.2802987
GATCTTT49400.025.0835746
TCTTTTT46200.024.8468178
CTTTTTT48700.023.6425049
TTTCGGA8700.023.3357561
CGATCTT82150.021.7307995
TAGGACA2600.021.5406974
CGATCTG99450.020.871955
TCGTATG2402.3646862E-1120.41645840
ACTAGTA1601.1856373E-619.6901042
CCGATCT294900.019.6680284
TTCCGAT294100.019.6387772
GATCTGG43000.019.3741026
ATTAGGA1451.1164435E-519.3128992
TCCGATC302750.019.1927413
CTAATCT8300.018.9751770
GTATCAT1301.0571987E-418.848111
ATCTGTG7100.018.7311387
GTATATA1702.1189717E-618.5313341
TTCGGAA11550.018.4872932
Produced by FastQC (version 0.11.5)