FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161132
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC18720.16122197992993045Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA16230.13977738965078906Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC15380.1324569471860219No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC14850.12789243600210828No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC13160.11333767392510068No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTA400.0024182935.00340334
GTATAGT450.00430552331.1154771
GTCCATA657.9528656E-426.9268531
TTTCGGA3200.026.2536811
AGTGTTG1001.3981917E-524.50132870
CGTGTGG25800.024.41992870
ACGTTTT1601.8626451E-924.06380369
GTACAAA750.001831546323.3366071
ATATCTC750.001833842423.33158355
TAGTTCT750.001834301923.33057658
GTACGTG1102.9203273E-522.2758521
ATCTTTT22800.021.9491627
TTCGGAA4000.021.878072
TCTTTTT22900.021.3948528
GACTGCA30450.021.38046670
CTCTAGG2501.8189894E-1221.0029451
TTACACT1003.9310814E-421.0020434
CTTTTTT23650.020.7163689
ATAGTAC850.003788652120.5902393
TAGTCAT850.003793381620.5858047
Produced by FastQC (version 0.11.5)