FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329290
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAACACCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACG20520.15436812132792693No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA14920.11224036891874609No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4000.029.7513581
ATCTGTG5150.025.1429967
CTATAGG1551.2896635E-924.8398441
CTAGTCG9800.024.64027420
CTCTAGG1601.8644641E-924.0635991
CGATCTG74550.023.5682665
GTCCGAT11150.023.54013411
GATCTGG30150.023.333526
TACTAAC10300.023.1078531
ATCTTTT31550.023.0720677
AGTTTAT11100.023.02166731
TCTTTTT31200.022.8822178
GATCTTT34650.022.7274556
ACACCGT11800.022.5425536
GATCCCT10900.022.47809215
ATCTGCG9700.022.0080037
TCTAGGG4950.021.9193692
CTAATCT5750.021.9132270
ATATACT800.002667239621.8751774
CTTTTTT32900.021.8062279
Produced by FastQC (version 0.11.5)