FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055573
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC13600.128839976013028Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA11400.10799821518739111Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA3400.030.8863031
GAGCATA1653.6379788E-1227.5792851
TTCGGAA4100.025.6106052
CGTGTGG18200.024.8096970
TCTTTTT31300.024.487318
TACGTCT1451.7293132E-824.1375896
ATCTTTT32450.024.0509077
CATACGT1652.6611815E-923.3330024
CTTTTTT33100.023.049949
GATCTTT37400.022.178836
CTCTAGG952.7787924E-422.108091
AGCATAC1604.908179E-821.8757252
CTATAGG1656.789378E-821.2148341
GATAATA1003.9349723E-420.99870764
GACTGCA29400.020.59689770
TATTACA850.003789834720.5889172
GTTATAC850.003791915520.58696764
GTCTAGG2603.6379788E-1220.1948911
GAGCGTA1408.254592E-619.9987711
GCTAATG1951.8240826E-819.7451870
Produced by FastQC (version 0.11.5)