FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335004
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC13760.10307085222216562Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCG4700.032.02494470
TATACTA851.3098217E-424.7040845
TTTCGGA3600.023.3325081
CAGTATC8650.023.06622769
ATCTTTT29400.021.6650912
GATCTTT31950.021.1408736
CTTTTTT30350.020.98851214
TAAGGTG4600.020.5419835
CGTGTGG12500.020.44234370
TATAATA1205.7201338E-520.4159452
TCTTTTT33250.019.78951513
ATAATAC1601.1875345E-619.6860663
TATAGTA2508.6765795E-1018.1993582
TATTACA1953.9523547E-717.9480842
ATACAGT1000.00969522917.4987286
TTATATA1401.8670005E-417.4987264
TAGGGGT4800.017.4987264
GTACAAG3053.8198777E-1117.2125071
TGTAGGG4150.016.8668752
GTCATAG2108.588104E-716.6660791
Produced by FastQC (version 0.11.5)