FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962716
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT42230.2151610319577565No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC34370.175114484214731No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT32460.16538307121356324No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC31850.16227513302994423No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG31270.1593200442651917No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA28890.14719399036844863No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA27330.13924582058739013No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG26700.13603598279119344No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT26490.13496603685912786No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA26410.13455843840881718No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT25030.12752736514095772No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT24980.12727261610951357No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA24370.12416467792589453No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG21770.11091772829079703No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA21000.10699459320655663No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA21000.10699459320655663No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGG20020.10200151219025065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG1652.6648195E-923.3332061
AGGGGTG8850.022.1467725
ACCGGTA9550.021.62181715
GTTAGAG11300.021.06075962
GTCCTAG10550.020.900361
CTAATCT10400.020.867470
CCGGTAA10150.020.68848816
CGTGTGG11550.020.30498970
CTCGCGT14350.019.99836329
ATATAAG11050.019.955152
ACGGTCC14400.019.9284159
AGGTTCG11350.019.73758767
GGTCCAA14250.019.64701511
GTCTTAC17250.019.2752591
GCTAATC12250.019.14470369
AGTTAGA12800.019.13954561
TAATACG11000.019.0888620
AGGACTA16700.019.071755
TAGAGGT11450.018.95041364
CAGGGGT4250.018.9410724
Produced by FastQC (version 0.11.5)