FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306955
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC15100.11553572999835496Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA13230.10122766277339311Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGG21250.026.84729470
ATTAGAC1056.77961E-726.6658783
GTACTAC1207.408198E-826.2512341
AGTATCG4300.024.41881870
TTTCGGA4400.023.8647581
ATCTTTT24850.023.3786967
CTAGGCG750.001833887223.3317539
TCTTTTT24250.023.235548
CTTTTTT24550.022.9516019
GATCTTT29950.021.5018716
AGTTACT850.003790846320.58841516
ATATACT850.003791686620.5876274
TTCGGAA6100.019.507622
TAACACT900.005278143619.443874
CAATACT900.005278143619.443874
CGTATAT900.00527931119.44312710
GTCTAGG3250.019.3855291
TCTAGGG5750.018.8690092
CAGTATC7300.018.69879369
TATAGTA1501.4971085E-518.6661172
Produced by FastQC (version 0.11.5)