FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3366929
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC82320.24449580017873854No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC76100.22602199214774055Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC74170.22028976554005147No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA69860.2074887828047458Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC62480.1855696986779347No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC57350.17033326215076114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA8850.030.0576171
AGTATCG11750.029.78967370
CAGTATG3300.026.51732370
CGTGTGG79850.025.60009470
TCTTTTT45900.024.3996098
CTTTTTT47600.023.601379
ATCTTTT50000.023.16887
TTCGGAA11750.022.9366862
GACCGAT118450.021.95620770
AGACCGA132850.020.31374569
CAGTATC21050.020.12011369
AACCTAT1408.259531E-619.9998576
GATATGT5300.019.8154
CTCTAGG4950.019.7987171
AGCATAC3850.019.091342
GATCTTT67300.018.9820336
ATATTAC5650.018.5850467
CAATACT950.007213103618.4211944
GAGACGA25500.018.39229866
TAAGTGT2104.276808E-818.33429168
Produced by FastQC (version 0.11.5)