FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847872
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC61390.33221998060471725No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC58420.31614743878363866No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC47670.25797241367367435No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC46270.25039613133377203Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC44230.23935640563848576No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA42080.22772140061649293Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCAGGGAGACGTGTGCTCTTCCGATCTGTGCAGGGAGACGTGTGCTCT18810.1017927648668306Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGT904.5092747E-935.0028370
ACGATTA802.468123E-630.62747646
TCCTAAT853.9607203E-628.8235192
AGTATCG6100.026.96939370
CGTGTGG55000.026.34758470
TTAGTGT2000.026.2507023
TATTGCA851.3094442E-424.7058732
TGTGTCA2001.8189894E-1224.49800310
GAGCATA1752.0190782E-1024.000641
GACCGAT62700.023.89347870
GACCTTT1953.092282E-1123.33395668
TACACCA2900.022.9316465
TAGTGTT2450.022.8577524
ATAAGTG1854.0381565E-1022.70330667
AGCATAC1854.0381565E-1022.7026962
ATATGTC1102.92532E-522.27211655
ACTGTGT2550.021.9595898
TACGTGT5350.021.5893668
GTTATTT1304.350346E-621.53670564
GTGTCAA2451.8189894E-1221.42682511
Produced by FastQC (version 0.11.5)