FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4413637
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC49800.11283211555458683Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA44590.10102779181885596Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGG68050.021.96223470
ATCTTTT78300.021.0521557
TCTTTTT77750.020.9326613
TTTCGGA13850.020.4697251
CTTTTTT79350.020.3782514
AGTATCG13600.020.3312970
GATCTTT97300.018.6326246
TATACTA2357.954441E-917.871895
TTCGGAA17550.017.749242
TAGGGGT13750.017.5631924
GACCGAT130650.017.30612670
GTATAAG4900.017.1432021
CAGTATC25800.016.95770569
AGCATAC4750.016.9473252
CTCTAGG8250.016.5457881
AGGGGTG34100.015.90868955
CATACGT5300.015.8490162
ATAGGGG17450.015.84487153
ACTGTGT4900.015.7131788
AGACCGA146750.015.6698269
Produced by FastQC (version 0.11.5)