FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455633
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTA20380.14000781790465042No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC17650.12125309057983709Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAATAGGCCCAGGCGACTGTTTAGCAAAAACACAGGTCTCTGCAAAACCG17370.11932952880293315No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC16050.11026130899752891No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA15820.10868124039507211Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCCTAAGGTGAGCGAGAGAACTCTCGTTAAGGAACTCGGCAAAATGACC15320.1052463086506008No Hit
GTACTATAATTTCACCGAGTCTCTCGTTGAGACAGTGCCCAGATCGTTAC15150.10407843185748056No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC15100.10373493868303343No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATA1001.3991494E-524.49987866
TCGTATA1352.3140274E-723.3348221
CATACGT1553.5093763E-822.5813084
CGTATAA1403.2872595E-722.5006642
TCTTTTT21750.022.20602613
TTCGGAA4450.022.023122
TACGGTA6400.021.87564536
CTATAGG4200.021.668051
ATCTTTT23150.021.61827912
AGGATTC7000.021.5006335
CTTTTTT22700.021.276714
TGTGTCG7250.021.24127426
CGTGTGG25450.021.04404670
TTAGGAT7750.020.7748053
GCGTAAC9250.020.05257652
GAGCATA1751.2652345E-720.0012761
TATTGTG1408.251629E-620.000592
TAGGATT7200.019.931144
ATCCTAA11650.019.8295921
CTATTGT1257.824354E-519.6012521
Produced by FastQC (version 0.11.5)