FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415550
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT28950.025.8709286
CGATCTG64950.025.2181645
TCTTTTT27550.024.8989628
ATCTTTT28000.024.8737817
CTTTTTT27950.024.5426259
CTATAGG1352.3156645E-723.3330151
ATACTAT750.00183338523.3330151
AGTATCG6100.022.9521370
TAGACTG1854.0381565E-1022.7015915
CTAGACT1403.289606E-722.4988984
TTCCGAT183300.022.43482
CTAGACA1253.094181E-622.3989054
CCGATCT186050.021.933894
GTGTATA800.002667659221.8747021
TCCGATC189850.021.8451253
CGATCTT49750.021.3858875
GATCTGG30000.021.1156336
GTATAGT1003.9346135E-420.9997141
TAGACAG2501.8189894E-1220.9989725
TCCTATA850.00379226820.5872272
Produced by FastQC (version 0.11.5)