FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346316
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCG5950.033.53222370
CTCTAGG1251.05501385E-1030.8025821
TATAGGA1101.017037E-625.4547882
GATCTTT41950.024.2791376
CAGTATC12000.023.91867369
ATCTTTT39500.023.746187
TAGTATA750.001833090823.3335574
TCTTTTT40750.022.8459978
CTTTTTT41750.022.2987889
ATCTGAG8400.022.0827267
CAGTATA1604.908179E-821.87683570
ATCTGGG13200.021.2115357
CGATCTG77200.021.1271445
GATCTGG29850.021.1057286
ATCTAGC5500.020.999427
TTTCGGA6700.020.3748421
CGATCTT69150.020.296655
TTCCGAT233850.020.1006852
CCGATCT235800.019.7860244
CAGTATG2504.1836756E-1119.60164370
Produced by FastQC (version 0.11.5)