FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255512
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC13640.10864093692453755Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA12900.10274692715003919Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA3900.033.2054861
TTCGGAA5300.023.7719482
CGTGTGG16800.023.54379770
GTTATTA1052.040651E-523.3335861
GACATCT6200.023.14725770
ATCTTTT28900.023.00879712
CATACGT1854.0381565E-1022.7011412
CTTTTTT29450.022.69973614
TCTTTTT29950.022.43763713
AGACATC7900.021.26774669
TCGTATG1003.9336213E-421.00022740
GCGTTTA1356.031063E-620.73848563
GTATTAT850.00379334320.58599965
CTAGGAC2051.4570105E-920.4863953
GATCTTT34600.020.330976
TCTAGGA2101.9663275E-919.9986252
GTTACGA2101.9663275E-919.99782864
ATCTGGG14200.019.962627
TCCTACA2002.4447218E-819.2486762
TGGGTCC1107.4811094E-419.0895946
Produced by FastQC (version 0.11.5)