FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566605
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC19840.1266432827675132Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA18820.12013238818974789Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC16750.10691910213487127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA1206.548362E-1132.08134
TTTCGGA5700.028.2465291
TCTTTTT49800.026.1429218
TTCGGAA6550.025.6480462
ATCTTTT51750.025.2254547
CTTTTTT52550.024.7748329
GATCTTT57100.023.9652046
TAGGGGT4800.022.6027344
CTAGGCC1755.2605174E-921.9986069
CGTGTGG25000.021.98281770
TGTAACA2301.2732926E-1121.3036772
GATTAGT1003.9361935E-420.99866915
CTATAGG1901.355329E-820.2638131
GTGTAAC2252.1100277E-1020.2228781
ATTCTAA1951.826811E-819.7442281
GGGTATT2705.456968E-1219.44321363
GACATCT7750.018.97017370
TAGAGTC1301.0576854E-418.84676437
ATAGTAC1301.05846266E-418.8449613
GGACTAG1501.4966201E-518.6672733
Produced by FastQC (version 0.11.5)