FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630879
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATTC1251.05501385E-1030.7998373
TACTAGT604.9826654E-429.1691972
TTTCGGA7350.027.621441
TTCGGAA8800.023.8657052
AGACATC11050.023.12346869
TCTTTTT47550.022.96447413
ATCTTTT48600.022.61027712
TAATACT1102.924548E-522.272614
GACATCT8500.022.2365470
ACCGTTA3150.022.219388
TTATTCC1755.2568794E-921.9998864
GATCTTT53050.021.903756
CTTTTTT50200.021.6127714
TGATATA1807.2832336E-921.38680863
TATACTA850.003791550620.5881275
TAGGGGT6650.019.4735814
GTCTAGG1801.702374E-719.446131
ATTAACT900.00527795519.4443424
CGTTATT2353.8016879E-1019.35922610
TCTAGGC3100.019.1910938
Produced by FastQC (version 0.11.5)