FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876134
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC23780.12675000826167002No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC22500.11992746786743379Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA21160.1127851208922177Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC20620.10990686166339932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT73950.025.0829417
GATCTTT78350.024.703026
TCTTTTT76450.024.0338088
TAGGGGT6850.024.014374
TTTCGGA7900.023.9244611
CTTTTTT77600.023.5868079
CTAATCT13350.021.49964170
GCTATAC1859.978976E-920.8106143
AGGGGTG21800.019.9080685
GCTAATC15550.019.80794169
CGTGTGG20950.019.54791670
GTATTGA1651.5939877E-619.0912361
CGATCTT138000.018.6664895
ACCGTTA3001.8189894E-1218.6654958
ACCTAGG3200.018.5940691
TTCGGAA10800.018.1489432
CATACGT2901.8189894E-1118.104242
TAATCTC8900.018.0906869
CTAGGGG5100.017.8429663
CAGGGGT8350.017.6046224
Produced by FastQC (version 0.11.5)