FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591749
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT19140.12024508889278399No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT16630.10447627107037605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT851.0655458E-732.9408234
GCCTAAT552.9936084E-431.8188381
TTTCGGA4900.028.5720181
TTCGGAA6250.025.1997282
TCCTACA3950.022.1516592
TATTACA952.782261E-422.1050242
AGTACTA952.782261E-422.1050245
ACTAGAC800.00266824721.87407765
ATCTTTT36050.021.7466557
ATAGACA5000.021.6997664
AGCATAC1957.8034645E-1021.538232
TCTTTTT35600.021.333378
TAGACAG7250.021.241155
GTCTAGG2650.021.1325111
CTTTTTT36050.021.0670729
GATCTTT40750.021.0433796
CTAGCTC1003.9348338E-420.9997734
AGTCCAA2700.020.73921422
GAGCATA2201.5643309E-1020.6822451
AGCTATA2603.6379788E-1220.1933634
Produced by FastQC (version 0.11.5)