FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944710
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT70520.23948028838153843No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT47470.16120432911899643No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG45380.15410685602317375No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA44580.1513901199099402No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC42580.14459827962685629No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA41720.14167778830513023No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT40340.13699141850980232No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT39860.1353613768418622No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG37430.12710929089791526No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT35670.1211324714488014No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA35610.12092871624030889No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC34560.11736300009168985No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA33500.11376332474165538No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG30770.10449246275524583No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA30460.10343972751136785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT29450.021.74714
GGTCCAA17000.021.40943711
CAGGGTT27750.021.19120439
AGGACTA29250.021.0582565
AGAAACG25950.020.90864267
GGAATCA28750.020.45516234
AATCAGG29100.020.44972236
TCAGGGT29000.020.3984938
TGCAACG29800.020.19914619
AACGGCT27250.020.16876870
AACGGGT29700.019.91298322
GAATCAG29550.019.90138235
CTTGTCT18500.019.8627059
GAGAAAC27500.019.85508566
TAACGGG30200.019.8153928
GGTAACG30100.019.76495726
GGGTTCG31150.019.438441
TCGCGTC18500.019.2965130
TATGCTT20350.019.261465
AACGGGG31100.019.01690329
Produced by FastQC (version 0.11.5)