FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650064
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAACACCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACG42610.2582324079550854No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTAT27520.16678140969077562No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA25930.15714541981401933No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAA23430.14199449233484276No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACT19500.11817723433757721No Hit
GCTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGC18870.11435920061282472No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGC18800.11393497464340778No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCT17670.10708675542281997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTGT9550.023.4545734
CTAGTCG14950.023.41044820
GAATAAT8300.023.1932621
ACTTGTG9750.022.9734545
CCTAGTC15300.022.8756119
TAGTTTA15150.022.63937230
GCGCTAT9900.022.27117311
GATCCCT15950.021.94337515
TGCGCTA10100.021.8301610
TACTAAC15750.021.778241
TCTACTT10150.021.723942
CATACGT1454.6115383E-721.723942
TTGTGCG10150.021.7226247
TTAGCCC1454.6142304E-721.72262411
TTTCGGA3550.021.69061
CTACTTG10200.021.6167953
AGTTTAT16150.021.45491231
ATAGTTT16500.021.42274929
ATCTTTT18600.021.07443812
CTATAGG1506.387527E-721.0004461
Produced by FastQC (version 0.11.5)