FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856730
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC9230.1077352258004272Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAT652.1015045E-532.3119471
GAGTAAT703.49085E-529.998764
GTATACG500.007210887527.99878714
TTTCGGA2400.027.7119851
GTATAGT906.1668325E-627.225811
ATAACTT2200.025.4564134
TATAACT2100.025.0018353
CATTAGC851.3085063E-424.7062536
CGTGTGG11200.023.43785370
ATAAAGT1052.0386837E-523.3350453
TAGGATT901.928881E-423.33232327
ATCTTTT17250.022.926547
TATTAGC1403.2807475E-722.5029662
TCTTTTT17750.022.2807258
ACATTAG952.779225E-422.1068865
GATGTAT952.7814205E-422.10430511
CTTTTTT18250.021.6702949
ATTAGCT1304.340014E-621.5400433
GATCTTT20450.021.2228136
GTACTAT1506.373357E-721.0027681
Produced by FastQC (version 0.11.5)