FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729936
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC30050.17370584807761674Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA28130.16260717159478733Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC27500.1589654183738589No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC24640.1424330148629776No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC19140.11063993118820581No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC17700.10231592382608373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4400.028.6375221
ATCTTTT34800.025.1425157
TCTTTTT34900.024.7696278
ACTCTCG851.3098048E-424.70473921
CTTTTTT35350.024.1572979
GATCTTT42550.022.290396
AGCATAC2207.2759576E-1222.272342
CGTGTGG34200.021.90210370
GAGCATA2301.2732926E-1121.305211
TCCTATA1154.1216066E-521.3039782
GACACTG70100.020.52210670
CTAGGAC1951.8280844E-819.7432483
TTCGGAA6400.019.6871592
GAGACGA12750.019.49042166
ACGAGTT2900.019.31168669
AGCGATC5650.019.20487269
CGTGTTG7400.018.44722670
AGACACT78700.018.32401569
GTATACG1351.4125265E-418.1488821
AACGTAT1953.952082E-717.94892519
Produced by FastQC (version 0.11.5)