FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186744
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.1520125654732613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA7550.029.6728381
TTCGGAA8350.026.408492
ATCTTTT68500.025.495467
TCTTTTT69500.025.128628
GATCTTT72150.025.0798786
CTTTTTT73200.023.8584569
ATGCTCT8150.022.7637771
GTGGGAT2350.022.33964762
GATCTGG37000.021.7569146
CGATCTT103400.021.66365
ATCTGTG7250.021.2406377
CGATCTG94450.020.9000995
AGCATAC1859.962605E-920.8118362
ATCTGGG15250.020.6550167
ATTAGAC850.00379067820.5883833
GGCTAGT850.00379067820.58838338
ATACAGA3100.020.3227276
TCTGTGC5000.020.2992928
CAGGGGT8900.020.0563244
ATCTGGC13200.019.6205277
Produced by FastQC (version 0.11.5)