FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548402
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC17350.11205100484241173Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA17100.11043643704929339Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC604.9866573E-429.1650923
TTTCGGA4750.025.7880841
CGTGTGG21050.025.77557470
TTCGGAA5400.022.6839622
ATACTAT800.002668397321.873821
TCTTTTT30350.021.1026528
ATCTTTT30850.020.9875227
CTTTTTT31700.020.0935529
GATCTTT37000.020.052976
GTATATA1055.469726E-419.998921
TATAGGC1257.831847E-519.5989444
GACACTG60800.019.45995770
TAGACTC1107.481438E-419.089885
TAGTACT1107.481438E-419.089884
GTCTAGG2951.8189894E-1218.9820251
GTGTAGG5350.018.970941
CTAAGAC1702.1218402E-618.5284123
TTATATG950.0072139518.420064
TAACATT1150.001008598618.2598844
TAATACT1351.4134274E-418.147174
Produced by FastQC (version 0.11.5)