FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453542
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC15710.10808081225035121Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGG18550.024.90782770
GTATTAT901.928527E-423.334561
ATACAGT901.9294265E-423.3329526
TCTTTTT27200.023.0319218
ATCTTTT28350.022.0976457
CTTTTTT29050.021.4446989
TTAGAAT1807.2777766E-921.3878083
GATATGT1859.982614E-920.80904254
GATCTTT34350.020.6837986
TATTATT1205.7182253E-520.4170382
TAGGGGT5350.019.6251744
AATCTAC900.005275919619.4454671
ACACTAC1107.4775075E-419.09125530
ACTGTAT1107.4809697E-419.0899438
TTATTCC1651.594517E-619.0899434
GTATAAG1501.49623775E-518.6676481
CATACGT2254.631147E-918.6670062
TAGCATC1150.001007138318.26371644
AGCATAC1551.984879E-518.0648462
TAATGTA1551.9866424E-518.0629815
Produced by FastQC (version 0.11.5)