FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993645
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC33820.11297264705734981Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA30770.10278439828369763Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA9250.028.0009161
ATCTTTT61000.023.5791937
TCTTTTT62000.023.4246648
TTCGGAA11650.023.1334172
CGTGTGG39950.022.6058870
CTTTTTT64650.022.5186219
GTCTAGG5800.021.7248481
GATCTTT73500.021.3333196
GCCTAAT2155.5988494E-817.9075621
TATAGGA2155.5999408E-817.9072632
TCTAGGG13050.017.7014312
GACACTG103000.017.53601870
ACCGTTA11100.017.3403028
AGCATAC2702.417437E-916.8521212
TTACACG2301.2095552E-716.7391194
AGACACT110300.016.5023769
TATAATA2151.0986387E-616.2793312
CAGGGGT8450.016.1538334
TATTTTA5650.016.10591366
GTACAGG15300.016.0135941
Produced by FastQC (version 0.11.5)