FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047439
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC22060.10774435770736027Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC21800.106474478604735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT7050.024.3264454
ATCTTTT66800.023.8388567
TTTCGGA10200.023.6772461
GATCTTT72500.023.1731726
TCTTTTT68950.022.8417158
TTCGGAA11550.022.424432
AGGGGTG22650.022.0973175
CTTTTTT71350.022.0237989
GATCTGG49650.021.289726
ATCTGGG21100.020.5678887
CTAATCT19600.020.00212170
TACACTT1055.468254E-420.0001685
ATCTGAG11850.019.7882297
CGATCTT130550.019.1690625
CGATCTG157800.018.8974975
TAATATC4100.018.78156569
CGTGTGG15000.018.66864670
ATCTGTG10600.018.4898197
CTAGGGG6250.018.4801563
ATCTAGG7400.018.44527
Produced by FastQC (version 0.11.5)