FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794634
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA3550.031.5493811
TTCGGAA4150.028.6747782
GTGTACA1305.4042175E-926.923151
ACGGTAA1251.09928806E-725.20006835
AACGGTA1301.606295E-724.23083534
TCTAGGG2800.023.7500632
ATCTTTT14750.023.0155627
GATCTTT17050.022.1687556
TTTATAC952.7803087E-422.1053223
TGTAGGG1905.620677E-1022.1053222
TCTTTTT15450.021.7462658
ATTAATA1454.604808E-721.7241972
CTTTTTT15650.021.6919849
GTCTAGG1304.3420187E-621.5385191
CGTGTGG6900.021.30574670
GTATGCC1356.0206658E-620.74079742
TTATACC850.003789690320.5882914
GTAGACA850.003789690320.5882914
GTCCTAA2051.4533725E-920.4878621
AATATAT1205.7147292E-520.4167215
Produced by FastQC (version 0.11.5)