FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231448
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCTA552.9935894E-431.8181636
GATCTTT29000.023.293096
ATCTTTT27450.022.94801112
CTTTTTT28000.022.6231514
CGTTATT3100.022.5778810
TCTTTTT28100.022.41809513
GATCTGG23700.021.4134886
GTCTAGG1154.1185456E-521.3051991
CGATCTG68350.020.8933035
ATCTGAG6450.020.617637
ACCGTTA5850.020.339398
TATAAAG2101.9626896E-920.0008012
ATTGTTA3350.019.84912114
TTTCGGA6650.019.4744641
GTACAGT900.005274613419.44601442
CGATCTT51050.019.4033245
GATCTGA19600.019.2857046
GTGTAAG1852.2713357E-718.9196761
GTCCTAC1501.4960964E-518.6674161
ATCCATA2254.627509E-918.6674141
Produced by FastQC (version 0.11.5)