FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477586
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC28490.19281449607670892Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC25800.174609125966272No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA25120.17000702497181214Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC22490.1522077225961805No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC19440.13156594607691194No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC17080.11559394850790411No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG755.5918215E-527.9980269
ATCTTTT26300.024.7511047
GAGGGTA1151.4987891E-624.34693114
ACGATTG1451.729677E-824.13867869
TCTTTTT26300.024.0857528
TTTCGGA3000.023.3324761
CTTTTTT27350.023.033119
GATCTTT33300.022.9112976
GTATGCC2800.022.49993542
CGTGTGG22500.021.93401370
TTCGGAA3700.020.8100452
GTATATA1356.0284947E-620.7399791
GGGTATT9500.020.63082116
GACGATT1901.3551471E-820.26378468
TCGTATG2453.092282E-1119.99994340
CCTAGGG5700.019.6484012
AGGGGTG11200.019.3736325
TCTGTCC6950.019.13598827
TACGTGT1852.272318E-718.91950668
CTACAGG3700.018.9182241
Produced by FastQC (version 0.11.5)