FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175684
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC22410.19061244347971054No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC22010.18721016871880541Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA20480.17419646775834322Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC20050.1705390223903702No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC17040.144936904814559No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC16230.13804729842372612No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT400.0024197334.9991957
TTTCGGA2600.029.6147021
CGTGTGG27750.028.50748470
AGCATAC1305.4114935E-926.9224572
ACTTTAG1606.548362E-1126.2493921
TCTTTTT18800.026.0621198
ATCTTTT19200.025.7014377
GATATGT1401.1919838E-824.9983654
CTTTTTT19600.024.81989
GACTAGC34150.023.67743770
ATATGTA901.9297664E-423.33180455
ACTAAAC750.001833755223.3318023
TTCGGAA3500.021.9994952
GCATACG1807.2723196E-921.3883971
AGTCTAA1003.9343166E-420.9995161
CATACGT1709.323412E-820.587762
CGTAATT1709.327596E-820.58688517
GATCTTT27250.020.420686
AGACTAG40400.020.36103869
GAGCATA1558.751522E-720.3221131
Produced by FastQC (version 0.11.5)