FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277779
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC16910.1323390038496485Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA14650.11465206424585159Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACATAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGATGCCTGC13280.10393033537098356No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG657.9607096E-426.92256417
CTTTTTT25650.025.9234059
TCTTTTT26250.025.3308728
ATCTTTT26700.025.2971657
CGTGTGG16350.023.33471770
GATCTTT31600.022.3747446
GAATTAG1102.9242194E-522.27230527
GAGCATA1454.6016248E-721.7279761
CATACAG1506.380469E-721.0020665
TACACGT1506.380469E-721.0020664
CGACACT1356.028562E-620.73953461
TACAGGG9550.020.1590522
TAGCACT6700.019.85274
GACTAGC34050.019.63405670
AGTATAG1801.7014463E-719.4463585
GCCATTA900.005278955719.44331456
ACGATTG1651.5925325E-619.09204169
TAGGATT1301.0579087E-418.84579527
CAGCTAG1501.4979407E-518.664859
CTGTGAC1501.4979407E-518.664859
Produced by FastQC (version 0.11.5)