FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367384
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC17640.12900545859831622Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA15760.11525657752321221Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC657.9599157E-426.9231911
AGCATAC2100.026.6658022
CGTGTGG21600.024.79358370
ATCTTTT30750.024.583677
TCTTTTT30300.024.3712588
CTTTTTT31150.023.706239
TGCTAGT1052.0397738E-523.33513870
GTGGCTA750.001833942723.3317269
GTATAAG1553.509922E-822.580741
CATACGT2401.8189894E-1221.8742924
GATCTTT39050.021.4213456
GACTAGC41100.020.6950370
GTATATG1055.4671336E-420.0000821
ACGTTTG3900.019.74511769
ATTGTAC900.005279471619.4431028
GAGCATA2757.2759576E-1219.090991
TTAGGAC3700.018.9169223
GTACTAA1301.0577889E-418.8462331
TTTCGGA4850.018.7629641
GTCTATG1501.4967558E-518.6667461
Produced by FastQC (version 0.11.5)