FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024879
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG64300.3175498387804901No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT60380.29819065731828914No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA57240.2826835578817302No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG44850.22149471647441651No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA44360.2190748187916414No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT44320.21887727612365976No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC42640.21058048406843075No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT42200.20840751472063268No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC42110.207963043717674No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT39270.1939375142909774No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA33830.16707171144547403No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC31550.1558117793705204No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG31130.15373758135671317No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA30110.1487002433231813No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA29740.1468729736443511No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT29290.1446506186295576No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA28680.14163809294283758No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA28340.13995898026499362No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA27030.13348945788859481No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT27020.13344007222159943No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA27010.13339068655460398No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC25990.12835334852107214No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAA25300.12494573749838879No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACT25160.12425433816045303No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA24800.12247645414861827No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA24400.12050102746880186No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC23210.11462413309634797No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA22650.11185853574460498No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC22290.11008065173277021No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGT21870.10800645371896295No Hit
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG21690.10711751171304557No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA21410.10573471303717406No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA21320.10529024203421537No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAA21060.1040062146923347No Hit
AGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGT20470.10109246033960548No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGG20360.10054921800265595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATTA10950.024.92911525
TTTCGGA4650.024.8400061
CGTAAAT11050.024.70290223
AAATTAC11150.024.48316626
ATATAAG11450.024.1503282
TATGCTT19500.023.1539125
TTGTCTC19250.022.72453110
ATTACCC12100.022.56093428
AGAAACG20100.022.28917167
CGCGTAA12800.022.14631321
TCTTTTT24400.022.0880438
ACCGGTA12500.021.83844415
CTTGTCT20200.021.8290489
GTAAATT12550.021.75036424
ATAAGAA13050.021.456534
CTTTTTT25300.021.4401029
CGTGTGG19350.021.34636570
ATATGCT21500.021.3261684
TATATGC20300.021.2074813
GCGCGTA13550.021.1787820
Produced by FastQC (version 0.11.5)