FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268211
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT200.006946769552.499025
TATACGA200.006946769552.4990268
ACGCGTT200.006946769552.499026
TTTCGGA950.047.893841
AGGTATG350.001254163439.99925633
ACCGCTA350.001254163439.99925628
AGTCCAC400.002416360234.99934861
GGGTACC400.002416360234.99934816
CTAGCTA400.002416360234.9993484
TTCGGAA1300.034.9993442
GACCGTT552.98771E-431.8175897
TTGTCTG450.00430302331.11053365
ATACTGC450.00430302331.1105336
CAGAACT604.974773E-429.1661224
GTATGCC604.974773E-429.16612242
TTATTCC604.974773E-429.1661224
GGTAGCT500.00719477328.004749
GAAGAGT500.007201326527.999481
TCTGTTA657.9442165E-426.92257327
ATACGTC808.647471E-526.249513
Produced by FastQC (version 0.11.5)