FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621238
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGG18750.021.65412570
AGCATAC1656.794835E-821.2148592
ATCTTTT31300.021.1330037
TTTCGGA5750.020.6983241
CTTTTTT31900.020.6258059
TCTTTTT31750.020.6130228
GATCTTT37700.019.9615576
TTATACC1301.05717976E-418.8480054
CATTACT1702.118255E-618.5318052
ATTACAT1551.9837424E-518.0662923
CAGGGGT5250.018.001774
GTGTACA2155.5879354E-817.9092881
TTATACT1401.8648719E-417.501724
ACGTTTG3600.017.5006469
GACTAGC53650.016.9624170
TTAATAC1250.001761856116.8016513
GACGTTT4200.016.66727468
GCCTTAT2151.0965832E-616.2811721
CTAGGAC2803.9071892E-916.2515963
CGATCTT86750.015.7369035
Produced by FastQC (version 0.11.5)