FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620377
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC17140.10577785293175601Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA16360.1009641583409293Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGG25250.027.17284670
ATCTTTT37500.024.9172337
TCTTTTT37700.024.3209068
GAGCATA1601.8662831E-924.0620541
CTTTTTT38750.023.4812629
GATCTTT45450.022.4851386
TTTCGGA6200.020.3222051
TTACTCG1901.3560566E-820.2627834
TAGTACA1055.468569E-419.999634
AGCATAC1801.7048114E-719.4434852
TAGGACA1301.05812695E-418.8458064
CATACGT2254.632966E-918.666324
ACTGTAC950.007216470318.4190068
GACTAGC52900.018.06539370
GTGTAGT2751.6007107E-1017.8178521
TTCGGAA6900.017.7527472
GTGTAGG5600.017.4996761
TGTAGGG6500.017.229922
TTTACTC2259.408359E-817.1107943
GTATATG1854.753023E-617.0267121
Produced by FastQC (version 0.11.5)