FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917872
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27210.29644656335523906No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC13730.14958512733801663Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA12150.1323713981905974Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC10770.11733662209981348No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC9680.10546132794115083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA5400.046.02451
TTCGGAA6550.037.4074022
GTTACCG400.002418930935.00073625
ACTGTCC601.21701305E-534.996928
TGCTTGA1800.033.0544555
AATCTGG2150.032.562371
TAGGGGT5700.031.9322364
AATCTAT552.9907085E-431.8223171
TATGCTG552.9916645E-431.8205835
AATCTGA901.7560524E-731.1151561
GCTTGAA1800.031.11007156
AATCTAG802.465391E-630.6289791
GTATGCC2200.030.2262642
AACGTAT1052.02308E-830.00391
ATGCCGT1900.029.47751444
GTTTAGT604.986086E-429.164164
AATCTAA853.95385E-628.8272761
GAGATTA853.960564E-628.82099364
CTGTGCG853.960564E-628.8209939
GCCGTCT1950.028.72168246
Produced by FastQC (version 0.11.5)